摘要
目的:探讨多西紫杉醇对黑素瘤B16F10细胞增殖及凋亡的体外作用。方法:以不同浓度多西紫杉醇处理B16F10细胞,MTT法检测细胞增殖速度,倒置显微镜观察细胞形态变化,流式细胞仪检测细胞周期分布,TUNEL法检测原位细胞凋亡。结果:多西紫杉醇呈剂量和时间依赖性抑制细胞增殖。以多西紫杉醇10μmol/L处理B16F10细胞,24h即可出现细胞形态改变,G2/M期阻滞,但凋亡细胞数目增多不明显(P<0.05);作用48h凋亡细胞数目明显增多(P>0.05)。结论:多西紫杉醇具有抑制B16F10细胞增殖、诱导细胞凋亡等作用,细胞凋亡的发生迟于周期阻滞。
Objective： To investigate the inhibition of cell proliferation and induction of cell apoptosis by docetaxel on melanoma B16F10 cells in vitro. Methods： Mouse melanoma B16F10 cells were treated by docetaxel with different concentration. Inverted microscope was used to observe morphological changes and cell proliferation was assayed by MTF method. Cell cycle was analyzed by flow cytometry and cell apoptosis was determined by TUNEL method. Results： Docetaxel inhibited the proliferation of B16F10 cells in a dose - dependent and thne - dependent manner. After B16F10 cells were treated by docetaxel in a concentration of 10 μmol/L for 24 hs, morphological change was observed and G2/M phase was blocked, but the number of apoptotic cells did not increase significantly （ P 〈 0.05）. After 48 hs, the number of apoptotic cells increased significantly （ P 〉 0.05）. Conclusion： Docetaxel can inhibit proliferation and induce apoptosis in B16F10 cells. The occurrence of apoptosis is later than cell cycle stasis.
出处
《中国麻风皮肤病杂志》
2008年第10期764-766,共3页
China Journal of Leprosy and Skin Diseases
基金
江苏省六大高峰人才基金资助
