摘要
目的:探讨阿尔茨海默病(AD)与线粒体的细胞色素氧化酶亚基Ⅲ(COX3)和辅酶Ⅱ(ND2)基因突变的关联性。方法:采用聚合酶链反应-限制性片段长度多态性技术,检测符合国际疾病分类第10版(ICD-10)诊断标准的60例AD患者(患者组),10个AD家系,和60名正常老人(对照组)的基因型和基因频率。结果:患者组线粒体ND2的nt5460基因位点基因变异为G→A,变异率13.3%,家系中同时有基因变异G→A,但变异无统计学意义。患者组中线粒体COX3的nt9861基因位点无基因变异。患者组和对照组均未发现基因有T→C的变异。结论:AD患者很有可能存在ND2的nt5460位点发生G→A的基因突变,与COX3的nt9861位点T→C突变无关。
Objective： To investigate the relationship between cyclooxygenase-3 （COX3） and NADPH2 （ND2） gene in chondriogene and Alzheimer disease（AD）. Method：The genotypes was measured by poly- merase chain reaction and restriction fragment length polymorphism technique in 60 patients with AD accord with the diagnostic criteria of International Classification of Disease Tenth Revision（ICD-10） , 10 family with AD and 60 normal elderly people were controlled . Results：There were genovariation on chondriogene ND2 nt5460 gene locus in 60 patients with AD, the genovariation was G→A and the aberration rate was 13.3% , There were significant differences in genovariation （G→A） of patients with AD. There were genovariation （ G→A） in family with AD and the aberration rate was 13.3% , the genovariation was obviously variation but not statistically significant. There were not genovariation on cbondriogene COX3 nt9861 gene locus in 60 patients with AD. The genovariation （T→C）was not found neither in patients nor controls. Conclusion： There were genovariation on chondriogene ND2 nt5460 gene locus and it was G→A in AD. There is no relationship between COX3 genovariation（T→C） on nt9861 gene locus in chondriogene and AD.
出处
《临床精神医学杂志》
2008年第5期333-334,共2页
Journal of Clinical Psychiatry
