摘要
目的探讨糖尿病对缺血预适应(IPC)在大鼠缺血再灌注心肌保护作用中的影响。方法取糖尿病SD大鼠及非糖尿病SD大鼠各30只,分为非糖尿病对照组(A组)、非糖尿病缺血再灌注组(B组)、非糖尿病IPC组(C组)、糖尿病对照组(D组)、糖尿病缺血再灌注组(E组)、糖尿病IPC组(F组),每组10只。动物处死后建立离体心脏Langendorff灌注模型。对照组采用全心灌流90min,余不做任何处理;缺血再灌注组采用心脏平衡灌流30min后,缺血30min,再复灌30min;IPC组采用心脏平衡灌流10min,经2次缺血5min再灌注5min后,缺血30min,再复灌30min。比较各组复灌30min后心排血量(CO)、左室发展压(LVDP)、左室内压最大上升和下降速率(±dp/dtmax)的恢复率,检测缺血前及复灌30min后冠脉流出液中肌酸激酶(CK)的活性和心肌组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的含量,并计算心肌含水率。结果与非糖尿病缺血再灌注组比较,非糖尿病IPC组CK活性、MDA含量、心肌含水率均明显降低,SOD含量明显增加,CO、LVDP、+dp/dtmax、-dp/dtmax恢复率明显增加(P<0.05)。而糖尿病IPC组与糖尿病缺血再灌注组比较上述变化均不明显(P>0.05)。结论糖尿病可抑制IPC对大鼠缺血再灌注心肌的保护作用。
Objective To explore the influence of diabetes on the protective effect of ischemic preconditioning （IPC） on ischemic. reperfused （IR） myoeardium of rat. Methods Sixty male SD rats （30 with diabetes and 30 without diabetes） were divided into six groups （10 each） ： non-diabetic rats control group （group A）, non-diabetic rats IR group （group B）, non-diabetic rats IPC group （group C）, dia- betic rats control group （group D）, diabetic rats IR group （group E） and diabetic rats IPC group （group F）. The isolated heart perfusion Langendorff models were established after rats were sacrificed. Isolated hearts of the two control groups were undergone a 90rain perfusion without any other intervention; those of the two IR groups were undergone a 30rain equilibration period, a 30min isehemia and a 30min reperfusion; those of the two IPC groups were undergone a 10min equilibration, then elicited by two cycles of 5rain ischemia interspersed with 5min reperfusion prior to 30rain ischemia and a a0min reperfusion. The recovery rates of the left ventricular function, such as cardiac output （CO） and left ventricular developed pressure （LVDP）, and the maximum upstroke and decay velocities of left ventricular pressure （±dp/dtmax） were recorded. The activity of creatine kinase （CK） in coronary outflow, contents of malonyldialdehyde （MDA） and super- oxide dismutase （SOD） in myocardium were detected, and myocardial water ratio were assessed. Results In non diabetic rats IPC group, the activity of CK, content of MI）A and water ratio of myocardium declined significantly, while the content of SOD increased and the recovery rates of CO, LVDP and ±dp/dtmax rose significantly compared with that in non-diabetic rats IR group （P〈0. 05）. However, no significant difference was found between group E and group F when compared on those parameters （P〉0.05）. Conclusion Diabetes may inhibit the protective effect of ischemic preconditioning on ischemic reperfused rat heart.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2008年第10期1195-1197,共3页
Medical Journal of Chinese People's Liberation Army
关键词
糖尿病
缺血预适应
心肌再灌注损伤
diabetes mellitus
ischemic preconditioning
myocardial reperfusion injury
