摘要
目的观测P53、Noxa在血管性痴呆大鼠海马CA1区表达,探讨血管性痴呆的发病机制。方法经Morris水迷宫筛选出学习记忆能力处于正常值范围的雄性SD大鼠24只,随机分为假手术组和模型组(各12只),采用双侧颈总动脉结扎法制备血管性痴呆大鼠模型,手术后2个月用Morris水迷宫观测各组大鼠在空间学习记忆方面的变化,HE染色观察各组大鼠海马CA1区锥体细胞形态学变化,免疫荧光染色检测P53、Noxa在海马CA1区锥体细胞的表达。结果模型组大鼠相对假手术组大鼠平均逃避潜伏期延长(P<0.01),空间记忆能力减退(P<0.01),海马CA1区神经细胞凋亡明显,P53、Noxa在海马CA1区表达的阳性细胞数明显升高(P<0.05),且直线相关分析显示Noxa的表达与P53的表达呈正相关(P<0.01)。结论海马CA1区P53和Noxa的表达升高在血管性痴呆发病机制中起重要作用。
Objective To investigate expression of P53 and Noxa in hippocampal CA1 area of rats with vascular dementia（VD）, and to explore its pathogenesis. Methods Twenty-four adult SD rats were randomly divided into sham-operation group（ n = 12） and VD model group（ n = 12）. VD rat models were established by bilateral occlusion of common carotid arteries. Morris water maze was used for place navigation test and spatial probe test. Neuropathological changes in hippoeampus were observed with HE staining, and expression of P53, Noxa in hippocampal CA1 region was measured with immunofluorescence staining. Results The average laten- cy in VD model group was significantly longer than that in sham-operation group（ P 〈 0.01 ）. The spatial memory in VD model group showed a significant impairment in comparison with that in sham-operation group（P〈 0.01 ）. Apoptosis was obvious in hippocampal CA1 area of VD rats with HE staining. In VD model group, the expression of P53 and Noxa in hippocampal CA1 region was higher than that in sham-operation group. The expression of P53 was postively correlated to expression of Noxa. Conclusion The increasing expression of P53 and Noxa may play an important role in the pathogenesis of vascular dementia.
出处
《山西医科大学学报》
CAS
2008年第10期865-868,共4页
Journal of Shanxi Medical University
基金
福建省卫生厅青年教师基金资助项目(2005-2-40)
