摘要
目的:研究葛根素对酒精性肝损伤大鼠肝组织转化生长因子-β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)表达的调控作用,探讨葛根素对酒精性肝损伤的防治机制。方法:将75只SD大鼠随机分成5组:正常组10只、模型组20只(乙醇等混合液,2次/d)、阳性对照组15只(乙醇等混合液+复方鳖甲软肝片,1 g.kg-1.d-1)、葛根素大剂量组15只(乙醇等混合液+葛根素片1.5 g.kg-1.d-1)、葛根素小剂量组15只(乙醇等混合液+葛根素片0.75 g.kg-1.d-1),8周后抽取股静脉血并处死,观察肝脏组织病理变化,检测肝组织TGF-β1和α-SMA的表达。结果:模型组大鼠肝组织病理变化和肝纤维化积分与其他各组相比都有显著性改变(P<0.05);葛根素大、小剂量组肝组织TGF-β1和α-SMA表达水平明显低于模型组(P<0.01);且葛根素大剂量组α-SMA水平明显低于对照组(P<0.05),小剂量组与模型组相比无显著性差异。结论:酒精性肝损伤大鼠肝脏组织TGF-β1和α-SMA有不同程度的升高,葛根素对其有调控作用,葛根素对酒精性肝损伤有保护作用。
Objective： To investigate the regulatory effects of puerarin on the levels of transforming growthβ1 （ TGF-β1） and α-smooth muscle actin （α-SMA） in rats with aleohohc injury liver, and to probe into the prevention and cure mechanism of puerarin on alcoholic liver injury. Method： Seventy five SD rats were randomly divided into 5 groups：normal group 10 rats. model group 20 rats （ alcohol etc mixed liquor twice per day）, control group 15 rats （ alcohol etc mixed liquor ＋ complex biejia ruangan plan 1 g·kg^-1 · d^-1 ）, puerarin high dose group 15 rats （ lcohol etc mixed liquor ＋ puerarin tablet 1.5 g·kg^-1· d^-1 ）, puerarin low dose group 15 rats （lcohol etc mixed liquor ＋ puerarin pill 0. 75 g · kg^-1· d^-1 ）. After 8 weeks, femoral vein blood of the rats were taken out in all rats and then rats were sacrificed to obtain liver samples. Samples were collected to observe pathological change and to detect expression of TGF-β1 and α-SMA in liver tissue. Result： Pathological change and hepatic fibrosis integration of liver tissue in model group were significantly changed compared with other group （P 〈 0. 05 ）. Meanwhile the level of TGF-β1 and α-SMA in both high and low dose puerarin groups Were significantly decreased compared with model group （P 〈 0. 01 ）. Furthermore the level of α-SMA of puerarin high dose group were significantly decreased compared with control group （ P 〈 0.05 ）, whereas there was no significant difference between puerarin low dose group and model group. Conclusion： There was an ascend in the level of TGF-β1 and α-SMA expression in rats with alcoholic injury liver, and puerarin has regulatory effects on the expression of TGF-β1 and α-SMA.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第19期2245-2249,共5页
China Journal of Chinese Materia Medica
基金
浙江省中医药管理局项目(2006C164)
