摘要
目的：探讨急性肺损伤大鼠下丘脑内ｃ－ｆｏｓ基因的表达规律及其与肺损伤的关系，并观察氯丙嗪对该二者的影响。方法：用６％氯化铵腹腔注射（０．６ｍｌ／１００ｇ）复制大鼠急性肺损伤模型，用氯丙嗪（１０ｍｇ／ｋｇ）腹腔注射进行预防或治疗，然后用“ＳＰ”免疫组化法检测大鼠下丘脑内ｃ－ｆｏｓ基因的表达。结果：急性肺损伤大鼠下丘脑内有大量ｃ－ｆｏｓ基因的表达，且呈现一定的时空规律，氯丙嗪可选择性抑制ｃ－ｆｏｓ基因的表达并同时减轻肺损伤程度，预防作用明显优于治疗作用。结论：该模型急性肺损伤的发生与下丘脑内ｃ－ｆｏｓ基因表达增加有关，氯丙嗪对该模型肺损伤具有理想的预防作用和较好的治疗作用。其机理可能与抑制下丘脑内某些核团的ｃ－ｆｏｓ基因表达有关。提示神经因素与ＡＬＩ的关系应受重视。
Objective: To explore the expression of cfos protooncogene in the hypothalamus after acute lung injury (ALI) in rats and its relation to pathogenesis of ALI. Methods: After the model of ALI was established in rats with intraperitoneal injection of 6% ammonium chloride at a dosage of 0.6 ml/200g, the animals were prevented and treated with intraperitoneal injection of chlorpromazine (CPZ) at a dosage of 0.6 ml/100g. Then the expression of cfos gene in the rat hypothalamus was determined with 'SP' immunocytochemical assay. Results: Great degree of the expression of cfos gene was found in the hypothalamus in rats with ALI and it exhibited some features in space and time. CPZ selectively inhibited the expression of cfos gene and thus attenuated the severity of ALI. The preventive effects of CPZ were better than its therapeutic effects. Conclusion: The occurrence of ALI is associated with the increase of the expression of cfos gene in the hypothalamus and CPZ can exert preventive and therapeutic effects on ALI. The mechanism of CPZ's effects might be inhibition of the expression of cfos gene in some parts of the hypothalamus, which suggests that the relationship between neural factors and ALI must be paid close attention to.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第6期503-505,共3页
Journal of Third Military Medical University
