摘要
目的 :探讨急性肺损伤 (AL I)大鼠海马内 c fos基因的表达规律及其与急性肺损伤的关系 ,并观察氯丙嗪对二者的影响。方法 :用质量分数为 6 %的氯化铵腹腔注射 (0 .6 ml/ 10 0 g)复制大鼠 AL I模型 ,用氯丙嗪(10 mg/ kg)腹腔注射进行预防或治疗 ,然后用链酶卵白素 (SP)免疫组织化学法检测大鼠海马内 c fos基因的表达。结果 :AL I大鼠海马内有大量 c fos基因的表达 ,且呈现一定的时空规律 ,氯丙嗪可选择性抑制 c fos基因的表达并同时减轻肺损伤程度 ,预防作用明显优于治疗作用。结论 :氯化钠诱导的 AL I的发生与海马内c fos基因表达增加有关 ;氯丙嗪对该模型肺损伤具有理想的预防作用和较好的治疗作用 ,其机制可能与抑制海马内某些核团的 c
Objective:To investigate the expression of cfos protooncogene in the hippocampus after acute lung injury(ALI) in rats and its relation to pathogenesis of ALI.Methods:ALI was established in rats with intraperitoneal injection of 6% ammonium chloride at a dosage of 0 6 ml/100 g.The animals were divided into prevention and treatment groups.Intraperitoneal injection of chlorpromazine(CPZ) at a dosage of 10 mg/kg was given either 0 5 hour before or 0 5 hour after the induction of ALI.Then the expression of cfos gene in the rat hippocampus was determined with SP immunocytochemical assay.Results:A heavy expression of cfos gene was found in the hippocampus in rats with ALI and it exhibited some temopral features. CPZ selectively inhibited the expression of cfos gene and thus attenuated the severity of ALI.The preventive effects of CPZ were better than its therapeutic effects.Conclusions:The occurrence of ALI is associated with the increase in the expression of cfos gene in the hippocampus and CPZ can exert preventive and therapeutic effects on ALI.The mechanism of effects of CPZ might be inhibition of the expression of cfos gene in some parts of the hippocampus,suggesting that there might be a relationship between nervous system and ALI.
出处
《中国危重病急救医学》
CAS
CSCD
2002年第9期548-550,共3页
Chinese Critical Care Medicine
基金
军队科技进步三等奖 (No.2 0 0 15 5 2 3 )
