摘要
采用改进的平板培养法在国内首先建立了铜绿假单胞菌生物被膜（Ｂｉｏｆｉｌｍ，ＢＦ），利用银染法检测铜绿假单胞ＢＦ的存在，通过扫描电镜观察了它的形态结构，利用计算机图像处理分析系统对经不同浓度盐酸氟罗沙星作用后银染的铜绿假单胞菌ＢＦ进行了分析统计。结果表明，平均灰度和透光率均随着浓度的升高而呈上升趋势，组间差异非常显著（Ｐ＜０．００１），提示盐酸氟罗沙星对铜绿假单胞菌ＢＦ的抑制作用在一定抗菌药物浓度范围内随着抗菌药物浓度的升高而加强。此结果与电镜和银染后光镜观察结果相同。本文旨在为临床治疗细菌生物被膜相关感染提供翔实可靠的实验依据。
Bacterial biofilm (BF) induced by Pseudomonas aeruginosa was established, and was detected by a rapid method with BF stained with AgNO_3. Scanning electron microscope was used to study the morphology and structure of BF. The difference of the stained BF influenced by fleroxacin hydrochloride (FLRX) of 0, 1, 4,16×MIC was counted up by using computer image analysis with significance of P<0.001. Results showed that there existed concentrationdependent relationship between the effects of FLRX controlling BF and the different concentrations. It suggested that the detail invitro experiment data provided in this report would be beneficial to clinicians in the treatment of biofilm associated infections.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1997年第6期435-441,共7页
Chinese Journal of Antibiotics
基金
国家自然科学基金1997~1999资助项目
关键词
盐酸氟罗沙星
铜绿假单胞菌
细菌生物被膜
Fleroxacin hydrochloride, Pseudomonas aeruginosa, Bacterial biofilm, Biofilm associated infection
