摘要
采用高效液相色谱法（甲基莲心碱为内标）测定了血浆中莲心碱的浓度。以ＵｌｔｒａｓｐｈｅｒｅＳｉ为固定相、二氯甲烷－异丙醇－二乙胺（７５１５０．２，Ｖ／Ｖ）为流动相，检测波长为２８２ｎｍ。血样用氨－氯化铵缓冲液调ｐＨ１０后用乙醚提取。平均回收率９３．６％，ＲＳＤ为１．９％，最低检测浓度０．０２５ｍｇ／Ｌ。
A sensitive method of high performance liquid chromatography (HPLC) for determination of liensinine in plasma is reported. An Ultrasphere Si column, 250mm×4 6mm i.d. with dichloromethane isopropyl alcohol diethylamine (75∶25∶0 2, V/V ) as mobile phase at a flow rate of 1 0mL/min and UV detector at 282nm were used. Neferine was served as the internal standard. Liensinine in plasma was extracted with diethyl ether three times after adding ammonia ammonium chloride buffer (pH 10). Liensinine was completely separated from nefrine. The retention times of liensinine and neferine were 8 5 and 5 0 minutes, respectively. The calibration line of liensinine was linear in the range of 0 0625 5 0mg/L( r =0 9996). The mean recovery was 93 6% with RSD of 1 9%. The precision of intra day and inter day for liensinine was in the range of 1 4% 4 1% and the detectable limit was 0 025 mg/L based on a signal to noise ratio of 3. The results showed that the method was simple and sensitive.
出处
《色谱》
CAS
CSCD
北大核心
1997年第4期347-348,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱法
莲心碱
人血浆
high performace liquid chromatography, liensinine,human plasma
