摘要
目的：研究阿司匹林铜（ＣｕＡｓｐ）对血小板聚集性的影响及其机制．方法：用Ｂｏｒｎ氏法测定ＣｕＡｓｐ对兔血小板聚集性的影响．用荧光光度法和放射免疫法观察ＣｕＡｓｐ对兔血小板５羟色胺的释放和ＴＸＢ２的产生及血浆中ＴＸＢ２和６ｋｅｔｏＰＧＦ１α水平的影响．结果：ＣｕＡｓｐ体外呈浓度依赖性抑制花生四烯酸（ＡＡ）诱导的血小板聚集和５羟色胺的释放（ＩＣ５０分别为１７和１９μｍｏｌ·Ｌ－１，９５％可信限为９－３３和１０－３０μｍｏｌ·Ｌ－１），且抑制ＴＸＢ２的产生（Ｐ＜００５）．ＣｕＡｓｐ１０ｍｇ·ｋｇ－１灌胃选择性抑制ＡＡ诱导聚集．降低血浆ＴＸＢ２，同时升高６ｋｅｔｏＰＧＦ１α的水平（Ｐ＜００５）．结论：ＣｕＡｓｐ体内外均有比Ａｓｐ更强的抗血小板聚集作用．
IM: To study the inhibitory effects of copper aspirin complex (CuAsp) on platelet aggregation. METHODS: With adenosine diphosphate the effects of CuAsp on platelet aggregation in vitro or in vivo were investigated. Radioimmunoassay and fluoro￣photometry were used to measure throm￣box￣ane B 2 (TXB 2) generation from platelets, the levels of TXB 2 and of 6 keto PGF 1α in plasma and the platelet serotonin release reaction. RESULTS: In vitro , CuAsp inhibited arachidonic acid (AA) induced aggregation (IC 50 =17 μmol·L -1 , 95 % confidence limits: 9-33 μmol·L -1 ), the release of 5 HT (IC 50 =19 μmol·L -1 , 95 % confidence limits: 10-30 μmol·L -1 ), and TXB 2 generation from platelets ( P <0 05). CuAsp 10 mg·kg -1 ig selectively inhibited AA induced aggregation, and increased the 6 keto PGF 1α concentration in plasma while decreased that of TXB 2. CONCLUSION: CuAsp, in vitro or in vivo , shows more potent inhibitory effects on AA induced aggregation than aspirin (Asp), related to the inhibition of platelet cyclooxygenase and the release of active substances from platelets.
出处
《中国药理学报》
CSCD
1997年第4期358-362,共5页
Acta Pharmacologica Sinica
关键词
铜
阿司匹林
血小板聚集
copper
aspirin
platelet aggregtion
thromboxane A 2
epoprostenol
serotonin
arachidonic acid
adenosine diphosphate
platelet activating factor
