摘要
以瓜胶为原料，首先进行酸降解，再以氯磺酸为磺化试剂，在适当的条件下进行磺化反应，获得低聚瓜胶磺化衍生物（Degraded guar sulfate，DGS）。考察不同磺化条件对硫酸基含量的影响，确立较优磺化反应条件。红外光谱分析表明，该衍生物为新型酸性粘多糖。以低聚瓜胶磺化衍生物为净化剂，研究其选择清除血浆低密度脂蛋白（LDL）及纤维蛋白原（Fib）的性能。结果表明，在pH=5．15，净化剂浓度2500mg/L时，可使血浆总胆固醇下降60％～66％，低密度脂蛋白和极低密度脂蛋白下降76％--89％，纤维蛋白原下降接近100％，而对高密度脂蛋白及血浆总蛋白水平无显著变化。
Degraded guar was prepared by acid with guar as the main material, which was then brought into reaction with chlorosulfonic acid under proper conditions, the sulfonated degraded guar was obtained successfully. The effects of sulfonation conditions on the SO4^2- content were investigated, and the proper reaction conditions were determined. The results of infrared spectrometry showed that this sulfated derivative is a novel heparin-like polysaccharide. At the same time, the selective removal of low density lipoprotein （LDL） and fibrinogen（Fib） by degraded guar gum sulfate was studled. The experimental resuhs showed that degraded guar gum sulfate is a novel LDL/Fib purifying agent. When pH = 5.15 and the initial concentration of the degraded guar gum sulfate is 2 500 mg/L, the reduction percentages were about 60 % - 66 % for total cholesterol, about 76 % - 89 % for LDL and very low-density lipoproteins （VLDL）, and almost 100% for fibrinogen. There were no significant chang~ regarding the level of high-density lipoproteins and total proteins.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2008年第5期1135-1140,共6页
Journal of Biomedical Engineering
关键词
低聚瓜胶磺化衍生物
类肝素
低密度脂蛋白
纤维蛋白原
Degraded guar sulfate（DGS） Heparin-iike materials Low-density lipoprotein （LDL） Fibrinogen （ Fib ）
