摘要
目的探讨臭氧对大鼠神经病理性疼痛起镇痛作用的部位和所需的臭氧浓度。方法应用成年雄性SD大鼠建立部分坐骨神经损伤（SNI）模型，采用随机数字表法分四组．即手术局部组、坐骨神经组、L5脊神经组、联合组（手术局部和L5脊神经），每组6只，于对应部位注射50μg／mL臭氧。另外，取SNI模型建立后12d大鼠，采用随机数字表法分四组．每组6只．四个组分别以40μg／mL、50μg／mL、60μg/mL和70μg／mL臭氧浓度对联合注射部位处理。采用大鼠50％的机械刺激撤足阈值（PWT）为观察项目。结果联合组和L5脊神经组在臭氧干预后机械痛阈明显升高，与其他处理组相比差异有统计学意义（P〈0．05）。在联合部位注射不同浓度臭氧的处理后，不同浓度间痛阈改变差异无统计学意义（P〉0．05）。结论臭氧对神经病理性疼痛镇痛的作用部位在手术局部和L5脊神经处。40～70μg／mL的臭氧对神经病理性疼痛均有镇痛效果。
Objective To investigate administration to relieve neuropathic pain in rats. the optimal concentration and site for ozone Methods Twenty-four adult male rats with sciatic nerve injury （SNI） were randomized equally into 4 groups, and received ozone injections for analgesia at different sites, namely the operative site, the sciatic nerve, the L5 spinal nerve or the operative site in addition to the L5 spinal nerve. Another 4 groups of rats with SNI （n=6） had ozone injections at the concentrations of 40, 50, 60, or 70 μg/mL, all administered at both the operative site and the L5 spinal nerve. The analgesic effects of the ozone injections were evaluated using paw withdrawal threshold test in all the rats. Results In rats with ozone injection at both the operative field and the L5 spinal nerve, the mechanical pain threshold was significantly increased in comparison with that in rats with injections at other sites （P〈0.05）, but different doses of ozone simultaneously injected at the two sites did not produce significant differences in the pain threshold （P〉0.05）. Conclusion Ozone produces obvious analgesic effects in rats with SNI-induced neuropathic pain when injected at both the operative site and the L5 spinal nerve, and ozone within the concentration range from 40 to 70 μg/mL has similar analgesic effect.
出处
《中华神经医学杂志》
CAS
CSCD
2008年第10期985-987,共3页
Chinese Journal of Neuromedicine
