摘要
建立了气相色谱-电子捕获检测器同时测定硝基咪唑类药物含量的方法。样品用磷酸盐调节pH约为8．8，乙酸乙酯提取，无水硫酸钠脱水。采用CBP-1毛细管柱分离，电子捕获检测器检测。甲硝唑、替硝唑、奥硝唑分别在0．2～15μg／mL、5．3～53μg／mL、4．0～40μg／mL范围内与峰面积呈良好线性关系，相关系数r分别为0．9993、0．9996、0．9995。样品加标回收率在95．8％～101％之间，相对标准偏差在1．8％～3．4％之间。本法简便、快速，可用于片剂和注射液中硝基咪唑类药物含量的测定。
A method for the determination of nitroimidazole in preparations by gas chromatography with electron capture detector was established. Nitroimidazole was separated and indentified on a CBP-1 capillary column in 8 min. The linear range of the calibration curve were 0.2-15μg/mL （r=0. 9993）, 5.3-53 μg/mL （r = 0. 9996） and 4. 0 - 40 μg/mL （r = 0. 9995） for metronidazole, tindazole and ornidazole,respectively. The recovery and the relative standard deviation of this method were 95.8%- 101% and 1. 8% - 3. 4%, respectively. The method is simple, rapid and may be used for the determination of nitroimidazole in tablets and injection.
出处
《分析科学学报》
CAS
CSCD
2008年第5期586-588,共3页
Journal of Analytical Science
