摘要
目的：建立HPLC法测定神农香菊中绿原酸、木犀草素的含量的方法。方法：采用高效液相色谱法，DIONEX-AC-CLAIM．120C18（250mm×4．6mm，5μm）色谱柱；以磷酸盐缓冲溶液（pH2．5）-甲醇（梯度洗脱）为流动相；流速为0．5mL·min^-1;柱温为35℃；检测波长为350nm。结果：绿原酸在0．024-0．192μg范围内呈线形关系，r=0．9994，平均回收率为98．3％，RSD为1．2％；木犀草素在0．058～0．464μg范围内呈线形关系，r=0．9998，平均回收率为97．3％，RSD为1．8％。结论：本方法简便，重复性好，可用于神农香菊原药材质量标准。
OBJECTIVE To establish a method for the determination of chlorogenic acid and luteolin in dendranthema indicum with HPLC. METHODS DIONEX-ACCLAIM. 120 C18 column（250 mm × 4. 6 mm, 5 μm）was used, the mobile phase consisted of phosphate buffer solution （pH 2. 5） and methanol with gradient elution; flow rate was 0. 5 mL·min^-1 ;column temperature was 35℃ ; the detective wavelength was 350nm. RESULTS The linear range of chlorogenic acid was 0. 024 μg to 0. 192μg,r= 0. 999 4,the average recovery was 98. 3% with RSD of 1.2%. The range of luteolin was 0. 058 μg to 0. 464μg, r = 0. 999 8, the average recovery was 97. 3 %, RSD was 1.8 %. CONCLUSION The method is stable, reliable, precise and can be used for the quality standard of Dendranthema indicum.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2008年第19期1629-1631,共3页
Chinese Journal of Hospital Pharmacy
基金
湖北省自然科学基金(编号:2004ABA242)
