摘要
测定９例健康青年２４小时血浆自由基清除剂超氧化物歧化酶（ＳＯＤ）及自由基代谢产物过氧化脂质（ＬＰＯ）的浓度变化，并应用时间生物学方法对所得数据进行分析，以探讨ＳＯＤ和丙二醛的节律变化特征。结果：血浆ＳＯＤ变化具有明显的近日节律特征（Ｐ＜０．００１），峰值相位（φ）在２１∶１７点，节律调整中值（Ｍ）为３９７５．３±６８０ｕ／ｇＨｂ，振幅（Ａ）为２７３．６２ｕ／ｇＨｂ，血浆ＬＰＯ变化也表现出近日节律特性（Ｐ＜０．０１，φ＝１５∶１５，Ｍ＝４．２３±０．８６μｍｏｌ／Ｌ，Ａ＝２．３７μｍｏｌ／Ｌ）。该研究结果为临床有关疾病的诊断。
Oxygen derived free radical system has been to be implicated in the pathogenesis of many diseases, such as cardiovascular disease, tumor, trauma and radiation injury. Many studies have suggested that oxygen free radical initiate a series of events that result in cell membrane alterations leading to the development of cell injury. The objective of the present study was to examine the circadian characteristics of some parameters of this system in thd blood. One parameter was superoxide dismutase (SOD), a major oxygen free radical scavenger; the other was malondiadehyde (MDA), an important product of lipid peroxidation initiated by oxygen free radicals.Blood samples were collected at 6h intervals beginning at 08∶00 over a 24 h span in nine healthy volunteers (5 females, 4 males, average age 30±5 years). All participants were synchronized for one week with diurnal activity from 07∶00 to 23∶00 and nocturnal rest, with three meals served at 07∶30, 12∶00 and 19∶00. Plasma SOD, MDA levels were measured by pyrogallol autoxidation assay, thiobarbituric acid spectrophotometry respectively, and the data were fitted with a 24 h cosine curve and analyzed further by a populationmean cosinor.The results showed that the plasma SOD levels exhibited a highly significant circadian rhythm (P<0.001) in healthy subjects, with acrophase (Φ) at 21∶17, mesor (M) of 3975.3±680.5 u/g Hb, and amplitude (A) of 273.62u/g Hb. A circadian rhythm of plasma MDA was also detected (P<0.01), Φ=15∶15, M=4.23±0.86μmol/L, A=2.37μmol/L). The evaluation of circadian natures of SOD and MDA may be important in the prevention, diagnosis and treatment of associated diseases.
出处
《华西医科大学学报》
CSCD
1997年第4期401-403,共3页
Journal of West China University of Medical Sciences
关键词
超氧化物歧化酶
过氧化脂质
血浆
节律变化
Circadian rhythm
Superoxide dismutase
Malondialdehyde
Plasma
Healthysubjects
