摘要
采用高胆固醇膳食诱发兔胆囊胆固醇结石模型，分别对高胆固醇膳食后１，２，３，４周组及对照组（每组１０只）血清脂质，载脂蛋白变化进行了研究。结果显示：①高胆固醇膳食后１周开始出现胆囊结石，２、３和４周组分别有４只、６只和７只兔出现胆囊结石；②血清总胆固醇、甘三油酯、磷脂、低密度脂蛋白胆固醇及极低密度脂蛋白胆固醇逐渐明显升高（与对照组比较Ｐ＜０．０５），高密度脂蛋白胆固醇及其亚组份有降低趋势，但无显著性差异（Ｐ＞０．０５）；③血清载脂蛋白ａｐｏＢ１００逐渐明显升高（Ｐ＜０．０５），以３、４周组明显；ａｐｏＡⅠ逐渐降低，以４周组最明显（Ｐ＜０．０５）；ａｐｏＣⅡ及ａｐｏＣⅢ逐渐明显升高，均在２周后最明显（Ｐ＜０．０５）。
In order to study the formation of cholesterol gallstone through rabbit model which was induced by high cholesterol diet(HCD), we investigated the rabbits serum lipoprotein cholesterols and apolipoprotein (apo) at 1 week (1w), 2 weeks (2w), 3 weeks (3w) and 4 weeks (4w) in comparison with those of a control group respectively. The results were as follows: (1) of 10 rabbits subjected to experiment, 4, 6, and 7 rabbits were found to have induced cholesterol gallstones in the 2w, 3w and 4w groups respectively. (2) The serum concentrations of total cholesterol (TC), triglyceride(TG), phospholipid (PL), low density lipoprotein cholesterol (LDLC) and very low density lipoprotein chloesterol (VLDL) increased significantly (1w, 2w, 3w and 4w groups vs control group, P<0.05), especially in the 3w and 4w groups; the surum concentrations of high density lipoprotein cholesterol and its subfractions (HDLC, HDL2C, HDL3C) decreased slightly (vs control group, P>0.05). (3) The serum contents of apoB100, apoCⅡ and apoCⅢ increased significantly (vs control group, P<0.05), especially in the 3w and 4w groups; the serum apoAⅠ reduced gradually in 1w, 2w and 3w groups, and decreased greatly in 4w group (vs control group, P<0.05). The results demonstrated that owing to the intake of high cholestrol diet with the passage of time, the increased concentrations of serum VLDLC, apoB, apoCⅡ and apoCⅢ possibly caused an enhanced secretion of biliary cholesterol into bile; that the decreased serum apoAⅠ level might reduce the secretion of antinucleating factor into bile. All of these factors and changes may play important roles during the formation of cholesterol gallstones.
出处
《华西医科大学学报》
CSCD
1997年第4期361-364,共4页
Journal of West China University of Medical Sciences
基金
卫生部科研基金
纽约中华医学基金会
关键词
血脂
载脂蛋白
动物模型
胆结石
胆固醇
Serum lipid Apolipoprotein Cholesterol gallstone Animal model
