摘要
H2O2能够氧化偶氮染料刚果红使之褪色,而模拟酶—血红蛋白对其具有催化作用。据此建立了一种以刚果红为指示剂的H2O2-刚果红-血红蛋白酶催化反应体系测定痕量H2O2的新方法。确定了反应的最佳条件,体系的酸度为pH10.7的NH3-NH4Cl缓冲溶液,最大吸收波长为497 nm。该法的线性范围为8.0×10^-8-8.0×10^-5mol/L,检出限为1.97×10^-9mol/L,表观摩尔吸光系数为4.6×10^4L.mol-1.cm^-1。该方法可用于雨水及消毒水中H2O2的测定。
A new enzyme catalytic method was developed for the determination of trace hydrogen peroxide using hemoglobin as a catalyst. Azo dye congo red is oxidized to fade by hydrogen peroxide because of producing hydroxyl radicals in alkaline medium, which is enhanced by hemoglobin. The optimum conditions and effect of foreign ions were dis- cussed. At pH 10.7 of NH3-NH4Cl buffer solution, the system exhibited maximum absorption wavelength at 497 nm. Under the optimal conditions, the degree of fading （AA） is linear to the H2O2 concentration （C） in the range of 8.0 ×10^-8 - 8.0 × 10^-5 mol/L, and the apparent molar absorptivity E497 was 4.6 × 10^4 L·mol^-1 ·cm^-1 with the detection limit of 1.97 ×10^-9 mol/L. This method is simple, sensitive and suitable for the determination of hydrogen peroxide in disinfectors with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第10期34-37,共4页
Chinese Journal of Analysis Laboratory
基金
河南省教育厅自然科学基金(2007150048,2008A150029)项目资助
关键词
催化光度法
H2O2
血红蛋白
刚果红
Catalytic spectrophotometry
Hydrogen peroxide
Hemoglobin
Congo red
