摘要
目的：检查转化生长因子β１（ＴＧＦβ１）、ＡＣＴＨ对碱性成纤维细胞生长因子（ｂＦＧＦ）受体的影响作用。方法：肾上腺皮质束、网状带细胞（ＢＡＣ细胞）的基础培养；１２５ＩｂＦＧＦ受体特异性结合标记；Ｓｃａｔｃｈａｒｄ分析。结果：２ｎｇ／ｍｌＴＧＦβ１处理细胞２４ｈ，每个细胞上高亲和力受体６５００个（ＫＤ＝３．１５１１×１０－１１ｍｏｌ／Ｌ），低亲和力受体数１４００００个（ＫＤ＝１．６２７７×１０－９ｍｏｌ／Ｌ）。ＡＣＴＨ处理组细胞的高、低亲和力受体分别是７６００和９５０００个／细胞（ＫＤ分别为３．６０９８×１０－１１ｍｏｌ／Ｌ和１．４０１２×１０－９ｍｏｌ／Ｌ）。结论：ＴＧＦβ１、ＡＣＴＨ均可增加ＢＡＣ细胞的ｂＦＧＦ受体数目；但二者在刺激强度和选择性上不同。ＴＧＦβ１、ＡＣＴＨ这对激素、因子对ｂＦＧＦ受体的不同调节作用可能与它们ＢＡＣ细胞不同的生物学作用有关。
Objective:To examine the influence of transforming growth factor β 1(TGFβ 1) and ACTH on basic fibroblast growth factor(bFGF) receptors of bovine adrenocortical fascilata reticularis cells (BAC cells).Methods:BAC cell culture in primary condition; 125 I bFGF receptor specific binding labeling;Scatchard analysis.Results:High and low affinity receptors were identified,for the cells that were treated with TGFβ 1(2 ng/ml),high and low affinity bFGF receptors were 6 500 sites/cell(K D=3.1511×10 -11 mol/L) and 140 000 sites/cell(K D=1.627 7×10 -9 mol/L) respeectively.For the cells that were treated with ACTH(3.0×10 -7 mol),high and low affinity bFGF receptors were 7 600 and 95 000 sites per cell respectively(K D=3.609 8×10 -11 mol/L and K D=1.401 2×10 -9 mol/L correspondingly).Conclusions:The number of bFGF receptors was increased under the influence of TGFβ 1 and ACTH,a detectable difference in strenth and selectivity was observed.The difference in effects on BAC cells may be due to their difference in biological activities between TGFβ 1 and ACTH.
出处
《蚌埠医学院学报》
CAS
1997年第6期367-368,共2页
Journal of Bengbu Medical College
关键词
TGFΒ1
细胞生长因子
CTH
BFGF
肾上腺
adrend corter
transforming growth factor β 1(TGFβ 1)
ACTH
basic fibroblast growth factor receptor(bFGF receptor)
Scatchard analysis
