摘要
背景:目前在纳米羟基磷灰石生物安全性实验中发现其引起红细胞聚集现象,是纳米羟基磷灰石血液相容性研究中亟待解决的问题。目的:探讨体外纳米羟基磷灰石引起红细胞聚集的机制。设计、时间及地点:体外细胞形态学观察实验,于2004-03/2006-06在武汉理工大学生物材料与工程研究中心实验室完成。材料:两种不同粒径羟基磷灰石粒子粉体由武汉理工大学生物医学材料与工程研究中心提供。方法:溶血实验评价材料对兔红细胞的影响,并对材料与红细胞共培养后做细胞形态学观察,Bialsche法检测纳米羟基磷灰石粒子与唾液酸的吸附量,绘制吸附等温线,纳米羟基磷灰石粒子与唾液酸共吸附作红外光谱分析。主要观察指标:纳米羟基磷灰石粒子对红细胞形态影响和超微结构观察,不同粒径的羟基磷灰石粒子对唾液酸的吸附量,纳米羟基磷灰石与唾液酸吸附后的红外光谱分析。结果:纳米羟基磷灰石粒子与兔红细胞共培养后可导致红细胞聚集,并且对细胞表面的唾液酸有强烈的吸附作用,红外光谱结果表明两者有明显的相互作用。结论:纳米粒子与红细胞体外共培养后,降低了细胞的Zeta电位和表面电荷密度,引起红细胞悬浮性下降,可能是导致聚集的发生的重要因素。
BACKGROUND： Currently, hydroxyapatite nanoparticles causing erythrocyte aggregation has been discovered on study of the biological safety, and it is in urgent need of resolving in its haemo-compatibility research. OBJECTIVE： Approaching the mechanism of hydroxyapatite nanoparticles causing erythrocyte aggregation in vitro. DESIGN, TIME AND SETTING： The experiment, a cell morphology in vitro, was performed at Biomedical Materials and Engineering Center of Wuhan University of Technology from March 2004 to June 2006. MATERIALS： Hydroxyapatite nanoparticles at two particle diameters were offered by Biomedical Materials and Engineering Center of Wuhan University of Technology. METHODS： The effect of hydroxyapatite nanoparticles on rabbit erythrocyte was evaluated with haemolysis test, and cell morphology was observed after the co-culture of the materials and erythrocyte. The amount of sialic acid adsorbed on hydroxyapatite nanoparticles was determined by Bialsche method, adsorption isotherm was drawn and both of them were analyzed by infrared MAIN OUTCOME MEASURES： Cellular morphology and ultramicrostructure after co-culturing hydroxyapatite nanoparticles with erythrocyte. Amount of sialic acid adsorbed on hydroxyapatite nanoparticles. Analysis of infrared spectrum of both. RESULTS： Hydroxyapatite nanoparticles could lead to erythrocyte aggregation after co-cultured. Moreover, a striking absorbability appeared between hydroxyapatite nanoparticles and sialic acid. Infrared spectra result indicated both of them had interaction obviously. CONCLUSION： Hydroxyapatite nanoparticles can degrade Zeta potential and surface charge density of erythrocyte while influenced erythrocyte suspending properties, it may be one of important factors for erythrocyte aggregation.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第36期7143-7146,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
