摘要
目的:了解慢性丙型肝炎患者血清非器官特异性自身抗体的发生率,分析自身免疫现象在慢性丙型肝炎病毒(HCV)感染中的意义.方法:回顾性分析临床诊断为慢性丙型肝炎患者93例,以同期住院的年龄及性别相匹配的慢性乙型肝炎患者80例作为对照.采用间接免疫荧光法测定血清非器官特异性自身抗体,ELISA测定抗HCVIgG、IgM,荧光定量PCR测定HCV-RNA,电化学发光法测定HBV-M,并常规测定ALT、AST及胆红素.结果:慢性丙型肝炎患者自身抗体阳性总检出率明显高于慢性乙型肝炎患者(48.4%vs7.5%,P<0.05).在检出的各种自身抗体中,ANA占73.3%(33/45),SMA占15.6%,AMA占6.7%,LKM占4.4%.慢性丙型肝炎患者HCV RNA阳性者66例,占71%.HCV RNA阳性组,自身抗体的总检出率为50.0%,而HCV RNA阴性组,自身体抗的总检出率为44.4%.两者总检出率比较差异无统计学意义.慢性丙型肝炎患者自身抗体阳性与性别无明显关系,但与年龄、肝脏病变的程度有密切关系(t年龄=1.501,P=0.022;tALT=3.403,P=0.000;tAST=3.448,P=0.000;tTBIL=3.055,P=0.000).结论:检测自身抗体及其滴度对慢性丙型肝炎的临床治疗、判断预后具有一定意义.
AIM： To observe prevalence of serum autoantibodies in chronic hepatitis C virus infected （HCV-infected） patients and to explore its clinical significance. METHODS： Ninety-three chronic HCV-infected patients were retrospectively analyzed. Eighty chronic HBV-infected patients were taken as con-trols, comparable with age and gender to HCV-infected patients in the same hospitalization period in our hospital. The serum autoantibodies were determined using indirect immunofluores-cence. The anti-HCV IgG/IgM were tested using ELISA. HCV-RNA was determined using fluores-cence quantity PCR. The HBV-M was measured using electrochemical methods. The serum AST, ALT and BIL were measured routinely. RESULTS： The total positive detectable rate of autoantibody in chronic HCV-infected patients was 48.4%, which was remarkedly higher than that in chronic HBV-infected patients （P 〈 0.05）. Among the detected autoantibodies, ANA ac-counted for 73.3%（33/45）, SMA 15.6%, AMA 6.7% and LKM 4.4%. There were 66 chronic HCV-infected patients with RNA positive, ac-counting for 71%. The total detectable rate of autoantibodies in the HCV RNA positive group was 50.0%; whereas in the HCV RNA negative group, it was 44.4%, indicating no significant difference. Positive autoantibody of chronic HCV-infected patients was not correlated to pa-tients’ genders but closely correlated to their age and pathological changes of liver （tage = 1.501, P = 0.022; tALT = 3.403, P = 0.000; tAST = 3.448, P = 0.000; tTBIL = 3.055, P = 0.000）. CONCLUSION： Determination of serum auto-antibody plays a role in clinical treatment and its prognosis of hepatitis C.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第27期3103-3105,共3页
World Chinese Journal of Digestology
