摘要
为探讨微生物在胆固醇结石形成中的作用，作者从３０例胆汁细菌培养阴性的胆囊胆固醇结石中提取ＤＮＡ，应用套式聚合酶链反应（ＮＰ－ＰＣＲ）技术从中特异性地扩增细菌ＤＮＡ片段。结果显示２６例（８６．６７％）胆固醇结石中有细菌ＤＮＡ存在。此外，用１６ＳｒＲＮＡ基因序列对照分析鉴定细菌种类，８例（２６．６７％）为与大肠杆菌相似的ＤＮＡ片段；７例（２３．３３％）为痤疮丙酸杆菌型ＤＮＡ序列；２例（６．６７％）ＤＮＡ片段与化脓性链球菌相关；７例（２３．３３％）ＤＮＡ片段具有多样性，可能有多种细菌混合感染；另外２例（６．６７％）ＤＮＡ分子量较低，归类于其它未鉴定细菌。作者认为多数胆固醇结石内有细菌ＤＮＡ存在，但这些微生物的确切作用，有待于进一步深入研究。
To search for bacterial DNA sequences in cholesterol gallstones with negative bacterial culture.We used nested primers polymerase chain reaction (NPPCR) technique to amplify bacterial gene fragments were amplified in vitro from DNA extracted from chloesterol gallstones.Comparative 16S ribosomal RNA sequence analysis was used for elucidation of bacterial identification.The gallbladder gallstones of 30 patients were analysed.Bacterial DNA was found in the stones of 26 patients. There was no difference either in cholesterol and water content or in harboring bacterial DNA of gallstones.E.colirelated DNA fragments were found in the stones of 8 patients (2667%).Propionibacteria type DNA was found in the stones of 7 patients (2333%).Stones of 2 patients (667%) harbored bacterial gene fragments with similarity of Streptococcus pyogenes.A more heterogenous sequence collection was found in 7 patients (2333%) and could be assigned to the multiple bacterial infections.Another stones of 2 patients (667%) had bacterial DNA with lower molecularweight which might be related to some unidentified bacteria.The results suggested that most cholesterol gallstones harbor bacterial DNA.It is important to determine whether these microorganisms are innocent bystanders or active participants in cholesterol gallstone formation.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1997年第11期633-666,共34页
Chinese Journal of Surgery
基金
国家教委博士点基金
