摘要
作者运用气相色谱法和高效液相色谱法分离和检测了胆固醇结石患者和正常人胆囊胆汁内的游离脂肪酸和卵磷脂的分子种类的含量和构成，同时运用体外模拟胆汁添加游离脂肪酸对胆固醇结晶生长的实验，观察四种游离脂肪酸对胆固醇结晶生长的作用。结果发现在胆固醇结石患者（２５例）胆囊胆汁内总的游离脂肪酸的量和其中的软脂酸、硬脂酸、亚油酸和花生四烯酸的含量显著高于正常人（１５例）。胆固醇结石患者与正常人胆汁内的卵磷脂分子的构成基本类似。在体外模拟胆汁的添加实验中，软脂酸、油酸、亚油酸和花生四烯酸具有显著的促成核作用，为胆汁内的非蛋白促成核因子。我们认为，胆汁中游离脂肪酸的含量和分子构成的变化在胆石形成机制中有重要的作用。
AbstractUsing gas chromatography and high performanceliquid chromatography(HILC ),we examined free fattyacid and lecithin molecular species in gallbladder biles from patients with cholesterol gallstones. Effect offree fattv acids on cholesterol nucleation in model bile was studied by a sensitive cholesterol crystal growthassay. Compared to bile of controls,biles from pa-tients with gallstones had higher total free fatty acidlevel,more palmitic acid,more stearic acid,morelinoleic acid and arachidonic acid.The lecithin pattenwas similar in all. After free fatty acids were added tomodel bile,palmitic acid oleic,acid,linoleic acid andarachidonic acid had significant effect of pro-nucleat-ing,free fatty acids on non-protein pro-nucleating fac-tor. These data suggest that variations in quantitationand composition of free fatty acids are importanct inthe pathogenesis of cholesterol gallstone formation.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1995年第11期657-661,共5页
Chinese Journal of Surgery
