摘要
目的探讨辣椒素受体在椎间盘突出致坐骨神经痛发病机制中的作用,了解其在背根神经节的表达与分布特点。方法采用自体髓核移植至L4、L5神经节,建立根性神经痛的动物模型,应用Von Frey针丝对神经行为的改变进行检测,采用免疫组化和RT-PCR法检测VR1在神经节及神经根中的表达,并分析其与神经行为改变之间的相关性。结果自体髓核移植可以导致明显的机械刺激痛觉过敏现象,VR1在自体髓核移植术后4d表达开始增强(P<0.05),术后1周表达最强(P<0.01),术后3周逐渐恢复正常,与神经行为改变之间存在显著相关性。结论VR1作为一种重要的伤害感受性分子,参予了髓核组织所致根性神经痛的发生,VR1可能是椎间盘突出症治疗新的靶点。
Objective To investigate the role of VR1 in the mechanism of sciatica due to disc herniation,and the expression and distributing character of VR1 in DRGs. Methods Autologous nucleus pulposus was applied to the peripheral of the L4 and L5 nerve roots and DRGs. Based on this animal model, Von Frey filaments was used to quantify behavior test. Immunocytochemistry and RT- PCR was used to find out the expression changes of VR1. Results The protein and mRNA expression of VR1 increased significantly（P〈0.05）in DRGs, nerve roots at postoperative 4d, especially at postoperative 1 week（P〈0.01）. At postoperative 3 weeks, the expression restored gradually to normal. There was a positive correlation between the mRNA expression of VR1 and neurological behavior changes. Conclusion VR1 play an important role in the mechanism of sciatica caused by nucleus pulposus transplanted to DRGs,it may be an new target to treat lumbar disc hernination.
出处
《重庆医学》
CAS
CSCD
2008年第19期2168-2170,共3页
Chongqing medicine
关键词
髓核
坐骨神经痛
辣椒素受体
背根神经节
nucleus pulpusus
sciatica
vanilloid receptor
dorsal root ganglion
