摘要
以包含BC8241Ht衍生系在内的21个玉米自交系为试材,从DNA分子水平上分析和鉴定欧洲玉米种质BC8241Ht衍生系的遗传多样性与亲缘关系。选取27对SRAP标记引物进行基因组DNA扩增,扩增产生275条谱带,其中有131条带存在多态性,多态性条带比率为47.6%。结果表明:所用21个供试玉米自交系具有丰富的遗传多样性;基于SRAP分子标记的聚类分析将欧洲玉米种质BC8241Ht衍生出的10个玉米自交系聚为一大类,但在聚类树上处于同大类中的第一和第三亚类;欧洲玉米种质BC8241Ht的10个衍生系间存在较近的亲缘关系,但彼此间又表现出一定的遗传差异;在对该种质及其衍生系实施改良过程中,应尽量避免与旅大红骨、塘四平头、PN和西南地方种质混血,可分别用热带或亚热带种质、Lancaster或Reid种质进行改良。
Analyzed and identified SRAP polymorphism and genetic relationship on derived lines from Europe maize germplasm BC8241Ht at DNA molecular level, 27 SRAP primer combinations were used to amplify the genome DNA of 21 maize lines of including derived lines from BC8241Ht. Total of 275 SRAP bans were observed and 131 out of these （47.6%） were polymorphic, and the polymorphism indicated that 21 maize lines used in the present study had abundant genetic diversity. The cluster analysis based on SRAP markers showed that 10 BC8241Ht derived lines were clustered in one group, but they were further clustered in first or third sub-groups. The close genetic relationship existed in 10 derived lines from Europe maize germplasm BC8241Ht, but the genetic differences also existed each other in the derived lines. In tile course of improving the germplasm and its derived lines, they should be avoided mixing with Lvdahonggu, Tangsipingtou, PN and Southwestern local germplasm, and it can be improved by introducing tropical, subtropical germplasm.
出处
《玉米科学》
CAS
CSCD
北大核心
2008年第5期16-19,共4页
Journal of Maize Sciences
基金
重庆市“十一五”水稻玉米重大专项(CSTC,2007AA1022)
重庆市自然科学基金项目(CSTC,2007BB1222)
关键词
衍生系
亲缘关系
SRAP标记
玉米种质
聚类分析
Derived lines
Genetic relationship
SRAP markers
Maize germplasm
Cluster analysis
