摘要
利用 RFL P和 SSR标记对 2 9个玉米自交系进行杂种优势群划分 ,筛选出 56个多态性 RFL P探针酶组合 ,6 6对多态性 SSR引物 ,分别在供试材料中检测到 187个和 2 32个等位基因变异。两种方法比较表明 ,SSR标记的平均多态性信息量 (PIC,0 .54)高于 RFLP(0 .4 2 ) ;但对供试材料的遗传多样性评价基本一致 ,平均遗传相似系数 (GS)分别为 0 .6 4和 0 .6 2。综合 RFL P和 SSR分析结果进行聚类分析 ,将供试材料划分为四平头 ,旅大红骨 ,L SC,BSSS和 PA五个类群 ,划分结果与系谱分析基本一致 ,并把系谱来源不清的种质划分到相应的杂种优势群。其中 PN群的确认 ,进一步完善了我国玉米种质杂种优势群的基本框架 ,为育种实践提供了有价值的信息。
Five heterotic groups were determined among 29 maize inbred lines using RFLP and SSR markers. A total of 56 polymorphic probe enzyme combinations and 66 polymorphic SSR primers were identified among the inbred lines, which produced 187 and 232 alleles respectively. Mean polymorphism information content (PIC) for RFLPs(0.42) was lower than that for SSRs(0.54), but mean genetic similarity (GS) were similar (0.62 & 0.64). The five heterotic groups based on both RFLPs and SSRs were Sipingtou, Luda Red cob, LSC, BSSS, and PA groups. They were consistent with the grouping based on the available pedigree data. Moreover, inbreds with unknown pedigree were assigned to the established heterotic groups. The identification of PA group provided a valuable guidance for maize hybrid breeding.
出处
《作物学报》
CAS
CSCD
北大核心
2001年第2期149-156,共8页
Acta Agronomica Sinica
基金
"九五"国家重点科技攻关项目! ( 96-0 0 2 -0 2 -0 5-4 )
亚洲玉米生物技术协作网基金! ( AMBIONET)
关键词
玉米
RFLP
SSR
遗传相似系数
杂种优势群
自交系
划分
Maize( Zea mays L.)
RFLPs (Restriction Fragment Length Polymorphisms)
SSRs(Simple Sequence Repeat)
Genetic similarity
Heterotic groups
