摘要
目的:研究自发性高血压♂大鼠(SHR)与WKY大鼠间的血压、可溶性细胞间黏附分子(sICAM-1)和纤溶酶原激活物抑制剂-1(PAI-1)表达的变化以及厄贝沙坦(irbesartan)和依那普利(enalapril)对SHR大鼠对上述指标的影响。方法:将30只♂SHR随机分为3组,每组10只。一组为SHR-I组,每日灌喂厄贝沙坦30mg.kg-1。一组为SHR-E组,每日灌喂依那普利10mg.kg-1。另一组为SHR空白对照组,不给予药物,正常饮水。另取10只WKY为正常对照组与上述3组比较。以上各组都喂养14周。用酶联免疫吸附法(ELISA)和发色底物显色法分别对4组大鼠血浆sICAM-1和PAI-1的表达进行检测。结果:SHR大鼠的血压、sICAM-1和PAI-1的表达明显高于WKY大鼠。厄贝沙坦组和依那普利组的血压、sICAM-1和PAI-1都明显低于SHR空白对照组,但两治疗组间差异无显性。结论:SHR♂大鼠的血压、sICAM-1、PAI-1表达都显著高于WKY大鼠,厄贝沙坦和依那普利都能明显地降低SHR大鼠的血压、sICAM-1、PAI-1表达。
OBJECTIVE To investigate the blood pressure , the level of plasma soluble intercellular adhesion molecule-1 （sICAM-1） and activity of plasminogen activitor inhibitor-1 （PAI-1） in spontaneouly hypertensive rats （SHR） compared with Wistar Kyoto（WKY） rats,and the effect of irbesartan and enalapril in SHR. METHODS Thirty SHR rate were randomly divided into 3 groups 10 rats each group , SHR-I group were fed with irbesartan of 30mg kg^-1 per day for 14 weeks. One group, SHR-E group were fed with enalapril of 10 mg kg^-1 per day for 14 weeks. While the remainder group （SHR control group） and 10 WKY were given normal water only. Using enzyme linked immunosorbent assay （ELISA） and spectrophotmetric assay to measure the activity of sICAM-1 and PAI-1 after treatment. RESULTS It was shown that the blood pressure, sICAM-1,PAI-1 lelvel of SHR control group were obviously heigher than WKY group （P〈0. 01 ）. After treatment of irbesartan and enalapril, the blood pressure, sICAM-1 and PAI-1 were significantly lower than SHR control group（P〈0.01）. There was no deference between SHR-Ⅰ and SHR-E. CONCLUSION The activity of sICAM-1 and PAI-1 are elevated in SHR. Irbesartan and enalapril not only decreased the blood pressure of SHR, but also decreased the acitviy of sICAm-1 and PAI-1. The deference between irbesartan and enalapril are not found in this invstigation.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2008年第17期1466-1468,共3页
Chinese Journal of Hospital Pharmacy
