摘要
目的本实验应用^60Co γ射线照射加液氮深低温保存大鼠同种带瓣主肺动脉（valved pulmonary homograft conduits，VPHC），并建立大鼠VPHC颈总动脉移植模型，观察不同照射剂量对同种带瓣主肺动脉免疫原性的作用，寻找合适照射剂量，为进一步基础研究及临床应用提供理论依据。方法1月龄封闭群Wistar大鼠50只作为供体鼠，雌雄不拘，随机分为单纯深低温保存组（实验对照组）、300Gy照射组、600Gy照射组、900Gy照射组和1200Gy照射组；成年雄性SD大鼠50只作为受体鼠，随机分为5组；另取10只SD大鼠行颈总动脉切断吻合术，作为空白对照组。应用流式细胞术对VPHC移植术后1周、2周、3周受体鼠外周血行CD4^＋T细胞及CD8^＋T细胞检测。结果照射组VPHC移植术后1周、2周、3周CD4^＋T细胞、CD8^＋T细胞百分率及CD4^＋／CD8^＋与空白对照组相比差异有统计学意义（P〈0．05），与实验对照组相比差异有统计学意义（P〈0．05）。结论应用^60Coγ对VPHC进行照射在保持VPHC活性的同时明显降低了其免疫原性。
Objective The method of keeping valved pulmonary homograft conduits（VPHC） by using ^60Co γirradiation and cryopreservation is used in this experiment and the transplantation model of VPHC is built. Different effects to the immunogenicity in VPHC rat caused by different dosage of ^60 Co iradiation are observed. To determine the suitable dosage of ^60Co γ used prior to cryopreservation in order to reduce the immunogenicity yet maintain the viability of VPHC. And to provide academic basis for further basic research and clinical application. Methods Fifty one-month old Wistar rats were used as donors and were divided into five groups （randomly,leave out sex ）. Fifty SD adult rats were used as recipients and were divided into five groups respectively. Four experimental groups were irradiated with ^60Co γ of different dosages prior to cryopreservation：300 Gy group, 600 Gy group, 900 Gy group, 1200 Gy group. The experimental control group received cryopreservation only. And ten SD rats underwent right carotid artery end-to-end anastomosis were used as a control group. Flow cytometry was used to test CD4^＋ ,CD8^＋ T cells in peripheral blood of recipient rats at weekly interval of 1,2, 3 weeks postoperatively. Results There is significant difference between experimental group and control group in percentages of CD4^＋ , CD8^＋ T cells and CD4^＋/CD8^＋ （ P 〈 0.05）. Conclusions ^60 Co γ irradiation can keep the activity of VPHC while reduce immunogenicity of VPHC at the same time in rats.
出处
《国际呼吸杂志》
2008年第18期1106-1108,共3页
International Journal of Respiration
