摘要
为探讨丙型肝炎病毒(HCV)肝外感染、复制场所与肝损害的关系,应用地高辛标记HCV RNA 探针原位杂交技术,对20例慢性丙型肝炎患者骨髓单个核细胞中的 HCV RNA(包括正链 RNA 和负链 RNA)进行了检测。结果有18例骨髓单个核细胞用正链 HCV RNA 探针杂交在较多细胞中出现杂交信号(90%),而所有患者骨髓单个核细胞用负链 HCV RNA 探针杂交无杂交信号出现。结果提示:慢性丙肝患者骨髓中存在 HCV RNA,即骨髓可受到丙肝病毒感染.但骨髓单个核细胞负链 RNA 阴性表明骨髓细胞并非 HCV 的复制场所。本研究对阐明 HCV 在人体中的致病作用以及丙肝的慢性化有一定的意义。
To investigate HCV extrahepiatic infection and replication site as well as its relation with liver damage,bone-marrow mononuclear cells from 20 cases of nutreated chronic hepatitis C virus infect- ed patients were studied by digoxin-labeled in situ hybridization.Positive HCV RNA stained were de- tected in 18 of 20 cases (90%).Negative HCV RNA stained were not detected in any cases.The results suggested that there was HCV RNA in the bone-marrow of the patients with hepatitis C and bone-mar- row could be infected by HCV.But bone-marrow cell is not the HCV replication site.This study has some significance in HCV elucidating the pathogenicity of as well as the pathogenesis of chronic hepatitis C.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1997年第4期200-202,共3页
Chinese Journal of Infectious Diseases
