摘要
周围神经损伤后缺损过长、过粗，由自体提供有限，移植后还会给供区带来神经功能抓害。以冷冻法降低异体种经移植后的抗原性，国内外已有报道，但其结果大相径庭。究竟哪一种温度和时间保存，排斥反应小，国内外尚未见肯定的意见、本实验研究，旨在用不同温度和时间保存异体神经进行移植以取得临床应用数据。实验取100只成年雄性SD大白鼠，体重250±20g，其中对照组异体神经不作任何处理共10只大白鼠。实验分3组，每组30只即A组为异体神经-196℃、－80℃、－40℃保存20分钟、每种温度均为10只大白鼠；B组为保存1周的同样温度和数量的大白鼠；C组为保存3周的同样温度和数量的大白鼠。手术在显微镜下由作者一人操作；来后不用任何药物，喂养条件、室温及管理条件完全一致。术后分别于6周和12周观察电生理变化，结果表明：同种异体神经可再生轴突，从电生理中发现，本实验组温度以-196℃为好。时间以3周为佳．为进一步在分子学上研究异体种经移植提供了统一的实验模型。
Because of the limitations in the use of autogenous nerve grafts for the rapair of peripheral nerve injuries, there has been muchinterest recently in the use of allogenous grafts,which were available in valying lengths and thicknesses, and which obviated the complication of sensory deficits of the donor area. However, the immune reaction elicited byallogenous grafts continued to be a serious problem. Cryopreservation of allogenous nerve graft can eliminate its postgrafting antigenicity.The optimum period as well as the best temperature range of cryopreservation had not been reported in the literature. One hundred male SD rats with body weight of 250±20g were used in this experiment which was carried out in 4 groups : (1) Control group: with the allogenous nerve given no treatmemt. (2) Group A:had the allogenous nerve grafts cryopreserved for 20 minutes with 10 animals each at -196℃, -80℃,and40℃. (3) Group B: with the same number of animals and temperature ranges, only the cryopreservtion period was prolonged to one week. (4) Group C:the cryopreservation was prolonged to 3 weeks.Theoperation was all done by the first author. The eletro physiological chahges were observed at 6 and 12weeks after the grafting. In general, the eletrophysiological chopes showed that the optimum condition for cryopreservation of the allogenous nerve grafts was 3 weeks at the temperature of -196℃.
出处
《伤残医学杂志》
1997年第3期6-9,共4页
Medical Journal of Trauma and Disability
