摘要
提出一种4.0代树枝状分子(4.0G-D)-卟啉(P)双发光分子新的磷光标记试剂(4.0G-D-P).基于4.0G-D-P标记伴刀豆凝集素(Con A)的产物(4.0G-D-P-Con A)能在聚酰胺素膜(PAM)上发射强而稳定的室温磷光(RTP),而且该标记产物能与甲胎蛋白异质体(AFP-V)发生特异性的亲和吸附(AA)反应,其反应产物保持了4.0G-D-P双发光分子RTP的优良特性,且ΔIp值与AFP-V的含量呈线性关系,加入Tween-80可以提高ΔIp值,据此建立了Tween-80-4.0G-D-P双发光分子标记Con A亲和吸附固体基质室温磷光法(AA-SS-RTP)测定人血清中AFP-V的新方法.直接法的检出限为0.31 pg/mL(4.0G-D)和0.43 pg/mL(P),灵敏度较高.无论用4.0G-D或P的激发/发射波长测定人血清中AFP-V的含量,结果与酶联免疫法(ELISA)相吻合.
The 4.0 generation dendrimer （4.0G-D）-porphyrin （P） dual luminescence molecule （4. 0G-D-P） is brought forward as a new phosphorescence label reagent. The product （4.0G-D-P-Con A） of 4.0G-D-P labeling concanavalin A （Con A） can emit strong and stable room temperature phosphorescence （RTP） signal on the surface of polyamide membrane （PAM） ,and it was also found that there is a specific affinity adsorptive （AA） reaction between 4. 0G-D-P-Con A and alpha-fetoprotein variant （AFP-V）. The product of the reaction can keep very well the RTP characteristics of 4. 0G-D-P, and the △Ip was linear correlation to the content of AFP-V. When adding Tween-80, the △Ip was increase. According to the facts above, a new method of affinity adsorption solid substrate-room temperature phosphorimetry for determination of AFP-V in human serum was established basing on Con A labeled with tween-80-4. 0G-D-P dual luminescence molecule. The detection limits of this method were 0. 31 pg/mL with 4.0G-D and 0.43 pg/ mL with P for direct assay. Determination of AFP-V in human serum with 4. 0G-D or P was as well as enzyme-linked immunosorbent assay （ELISA）, the sensitivity was obviously improved and the applicability was wider.
出处
《分析测试技术与仪器》
CAS
2008年第3期155-160,共6页
Analysis and Testing Technology and Instruments
基金
福建省自然科学基金计划资助项目(项目编号:2008J0313
D0510027)
