摘要
基于外重原子微扰剂LiAc存在下,罗丹明6G(R)-二溴荧光素(D)SiO2发光纳米微球(R-D-SiO2)在醋酸纤维素膜(ACM)上可分别发射R(λex/λem=481/648nm)和D(λex/λem=457/622nm)强而稳定的室温光信号.用R-D-SiO2标记麦胚凝集素(WGA),在ACM上采用R-D-SiO2-WGA-ALP(直接法)亲和吸附反应方式,研究了利用亲和吸附固体基质室温磷光法(AA-SS-RTP)测定碱性磷酸酶(ALP)的条件和分析性能.研究结果表明,对0.4ml点样量,R-D-SiO2-WGA-ALP法的检出限为0.41agspot-1(457/622nm)与0.44agspot-1(481/648nm).用本法和ELISA方法对比测定了血清试样中ALP的含量,结果相吻合.本研究表明,可分别采用D或R的磷光激发/发射波长进行测定,不仅提高了AA-SS-RTP的灵活性,而且适应性更广.
In the presence of heavy atom perturber LiAc, the silicon dioxide nanoparticle containing Rhodamine 6G （R） and dibromoluciferin （D） （R-D-SiO2） can emit strong and stable solid substrate-room temperature phosphorescence signal of R （λex/λem = 481/648nm） and D （λex/λem = 457/622 nm） on the surface of acetyl cellulose membrane （ACM）. R-D-SiO2 is used to label triticum vulgare lectin （WGA）. Then affinity adsorption reaction, R-D-SiO2-WGA-ALP （direct method） （ALP refers to alkaline phosphatase, are carried out on ACM. The conditions and the analytical characteristics for the determination of alkaline phosphatase （ALP） using affinity adsorption solid substrate-room temperature phosphorimetry （abbreviated to AA-SS-RTP） are studied. For 0.40 μl drop of sample, results show that the detection limit of direct method are 0.41 ag spot^-1 （457/622 nm） and 0.44 ag spot^-1 （481/648 nm）. The results of content of ALP in human serum are correlated well with those obtained by enzyme-link immunoassay. Simultaneously, either the phosphorescence excitation/emission wavelength of R or D in R-D-SiO2 is chosen to determine ALP, it can promote the agility and widen the adaptability of AA-SS-RTP.
出处
《漳州师范学院学报（自然科学版）》
2007年第2期76-82,共7页
Journal of ZhangZhou Teachers College（Natural Science)
基金
漳州师范学院基金资助项目(SK06003)
关键词
碱性磷酸酶
麦胚凝集素
罗丹明6G-二溴荧光素纳米微球
亲和吸附
固体基质室温烧光法
alkaline phosphatase
triticum vulgare lectin
Rhodanme 6G-dibromoluciferin nanoparticle
affinity adsorption
solid substrate-room temperature phosphorimetry
