摘要
作者以流产绒毛和外周血淋巴细胞DNA为材料,通过Southern印迹杂交和点杂交,考察了用生物素标记Y特异DNA探针PHY2.1和Cos84进行产前性别诊断的可行性。结果表明:PHY2.1可用于Sou-thern或点杂交,而Cos84只可用于Southern杂交进行产前性别诊断。
Using biotinylated human Y-specificDNA probe PHY2.1 and Cos 84, we carriedout Southern and dot hybridization withhuman DNA from artificially aborted cho-rionic villi.and with DNA of peripherallymphocytes from normal male and female.The results show that PHY2.1 may beapplied in prenatal sex identificationboth by Southern and by dot hybridizationbut with Cos 84,only Southern hybridi-zation could be used.
出处
《华西医科大学学报》
CAS
CSCD
1990年第3期277-280,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
国家计划生育委员会资助课题~~
关键词
产前性别诊断
DNA探针
生物素标记
prenatal sex identification
Y-specific DNA probe
Biotin-labeling
