摘要
高效液相色谱法测定牛黄解毒片中人工牛黄含量。采用Kromasil C18柱,以甲醇-三氯甲烷-0.1%磷酸溶液（81∶13∶6）为流动相;检测波长：450nm;流速：1mL·min^-1。胆红素在0.00525～0.0525μg范围内具有良好的线性关系,r=0.9998。平均回收率为101.44%,RSD=1.7%。本方法操作简单,重复性好,结果准确,可以控制本制剂质量。
Method of determination of artificial bezoar in Niuhuang Jiedu Tablets was established by HPLC.The Kromasil C18 column was used,and the mobile phase was composed of methanol-chloroform-0.1% phosphate solution（81∶13∶6）.The flow rate was 1.0 mL·min^-1.The determined wavelength was 450nm.It showed a good linear correlation within the concentration of 0.00525～0.0525μg,r=0.9998.The average recovery was 101.44%,RSD=1.7%.The method is accurate,simple and reproducible.It can be used in the quality control of this preparation.
出处
《中国药事》
CAS
2008年第9期803-805,共3页
Chinese Pharmaceutical Affairs
