摘要
从80个随机扩增多态性DNA(RAPD)引物中筛选出26个扩增稳定、带纹清晰的引物,对从玉米丝黑穗病发生严重的大部分北方省市收集分离的68个玉米丝轴黑粉菌菌株进行了DNA遗传多样性分析.结果表明,RAPD谱带多态性为98.33%,北方玉米丝轴黑粉菌具有丰富的遗传多样性,遗传分化明显;种群内遗传变异小于种群间遗传变异.玉米丝轴黑粉菌遗传分化除明显受地理阻隔影响外,也可能与玉米种子调运过程中携带病菌基因的遗传迁移相关.取相似系数为0.76阀值时的系统聚类分析,可将68个菌株划分为13个遗传宗谱.本研究结果完善和丰富了我国玉米丝轴黑粉菌遗传多样性评价研究,对玉米抗病资源筛选和抗病育种具有重要参考价值.
The genetic diversity of 68 isolates of Sporisorium reilianum in northern China was analyzed using 26 random amplified polymorphic DNA（RAPD） markers.By Popgene software analysis,the polymorphism rate was 98.33%.It indicated an abundant genetic diversity and a distinct differentiation among the populations of Sporisorium reilianum.And the genetic difference in populations was smaller than that among populations.The genetic differentiation was affected not only by geographical regions,but also probably by gene transference through pathogens carried by seeds.According to the data,a dendrogram of genetic relationship which was constructed using UPGMA method,showed that all the 68 populations were classified into 13 cluster groups with the similar coefficient of 0.76.This result had perfected and enriched the research on genetic diversity evaluation of Sporisorium reilianum in China,and provided significant reference for selection of disease resistance resource and breeding for disease-resistance.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2008年第9期846-853,共8页
Chinese Journal of Biochemistry and Molecular Biology
基金
北京农业育种基础研究创新平台课题资助(No.Yzpt02-06)~~
关键词
玉米
丝轴黑粉菌
遗传多样性
遗传分化
maize
Sporisorium reilianum
genetic diversity
genetic differentiation
