摘要
目的观察在不同浓度葡萄糖、雌二醇环境下人成骨肉瘤MG63细胞株肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其护骨素(OPG)、护骨素配体(OPGL)的表达,探讨绝经后糖尿病女性骨质疏松症的发病机制。方法用不同浓度葡萄糖(5.5、16.7、33.3 mmol/L)和17β-E2分别刺激培养的MG63细胞24 h,RT-PCR法检测TRAIL、OPG、OPGL mRNA的表达。结果葡萄糖对MG63细胞中TRAIL、OPGL mRNA的表达,均按照对照组、5.5 mmol/L组、16.7 mmol/L组、33.3 mmol/L组顺序递增(P<0.05),OPG mRNA的表达按照此顺序递减(P<0.05)。33.3 mmol/L组TRAIL mRNA的水平明显高于对照组[(1.004±0.070)vs(0.740±0.023),P<0.05],而17β-E2可增加OPG mRNA的表达,减少TRAIL、OPGL mRNA的表达。结论高糖环境可能导致成骨细胞中TRAIL和OPGL表达增多,OPG的表达减少,从而导致骨质疏松。而雌二醇对葡萄糖环境下的MG63细胞中上述因子的表达可产生一定的对抗效应。这可能是绝经后糖尿病女性骨质疏松症患者雌激素替代治疗的依据之一。
Objective To observe the regulative effects of different concentrations of glucose and 17β-estradiol on the expressions of Tumor necrosis Factor-related apoptosis-inducing ligand（ TRAIL）, osteoprotegefin（ OPG）, and the ligand of osteoprotegerin （OPGL） in osteosarcoma MG63 cells, and to study the role of non-physiological concentration of glucose and 17β-estradiol in pathogenesis of postmenopausal diabetic osteoporosis. Methods MG63 cells were incubated with glucose at the concentration of 5.5,16.7,33.3 mmol/L and 17β-estradiol for 24 h respectively. The expressions of TRAIL, OPG and OPGL mRNA were examined by reverse transcfiptase（RT）-PCR. Results The mRNA expressions of TRAIL and OPGL in MG63 cells increased in the order of control group, 5.5, 16.7,33.3 mmol/L glucose groups（ P 〈 0.05）,and the expression of OPG mRNA decreased in the same order（ P 〈 0.05 ）. TRAIL mRNA level in 33.3 mmol/L group was significantly higher than that in control group[ （1.004 ± 0.070）vs（0.740 ± 0.023 ）, P 〈 0.05], while 17β-estradiol could increase the express of OPG mRNA, and decrease TRAIL, OPGL mRNA in glucose environment. Conclusion High concentration of glucose could lead to the increasing expressions of some bone-resorbing cytokines such as TRAIL and OPGL in osteoblasts but the decreasing expression of OPG, which induced diabetic osteoporosis. 17β-estradiol could oppose the expressions of OPG, OPGL and TRAIL mRNA in high glucose concentration environment, which may be one of the key pathogenetic factors of 17β-estradiol replacement therapy in postmenopausal diabetic osteoporosis.
出处
《中国骨质疏松杂志》
CAS
CSCD
2008年第9期638-641,共4页
Chinese Journal of Osteoporosis
关键词
高糖
雌二醇
MG63细胞
肿瘤坏死因子相关凋亡诱导配体
High glucose concentration
17β-estradiol
MG63 cell
Tumor necrosis Factor-related apoptosisinducing ligand
