摘要
目的观察不同浓度葡萄糖环境下人成骨肉瘤MG63细胞株肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其护骨素(OPG)、护骨素配体(OPGL)的表达,探讨糖尿病骨质疏松症的发病机制.方法用不同浓度葡萄糖(5.5,16.7,33.3mmol/L)分别刺激培养的MG63细胞24h,RT-PCR法检测TRAIL,OPG,OPGLmRNA的表达,免疫组织化学法检测MG63细胞中TRAIL的表达及分布.结果葡萄糖对MG63细胞中TRAIL,OPGLmRNA的表达,均按照对照组、5.5mmol/L组、16.7mmol/L组、33.3mmol/L组顺序递增(P<0.05),OPGmRNA的表达按照此顺序递减(P<0.05).33.3mmol/L组TRAILmRNA的水平明显高于对照组[(1.004±0.070)vs(0.740±0.023),P<0.05],TRAIL免疫反应阳性物质密度也明显高于对照组.结论高糖环境可能导致成骨细胞中TRAIL和OPGL表达增多,OPG的表达减少,这可能是糖尿病骨质疏松症的重要发病机制之一.
AIM： To observe the regulative effects of different concentrations of glucose on the expressions of tumor necrosis factor-related apoptosis-inducing ligand （ TRAIL ）, osteoprotegerin （OPG） ,and the ligand of osteoprotegerin（OPGL） in osteosarcoma MG63 cells, and to study the role of non-physiological concentration of glucose in pathogenesis of diabetic osteoporosis. METHODS： MG63 cells were incubated with glucose at the concentration of 5.5, 16.7, 33.3 mmol/L for 24 h respectively. The expressions of TRAIL, OPG and OPGL mRNA were examined by reverse transcriptase（RT） -PCR. Expression and distribution of TRAIL in MG63 cells were investigated by immunohistochemical method. RESULTS： The mRNA expressions of TRAIL and OPGL in MG63 cells increased in the order of control group, 5.5, 16.7, 33.3 mmol/L glucose groups （ P 〈 0.05 ）, and the expression of OPG mRNA decreased in the same order （ P 〈 0.05 ）. TRAIL mRNA level in 33. 3 mmol/L group was significantly higher than that in control group[ （ 1. 004 ±0.070） vs （0.740 ± 0.023 ）, P 〈 0.05 ], and the intensity of immunostaining for TRAIL was stronger than that in control group. CONCLUSION： High concentration of glucose could lead to the increasing expression of some bone-resorbing cytokines such as TRAIL and OPGL in osteoblasts but the decreasing expression of OPG, which may be one of the key pathogenetic factors of diabetic osteoporosis.
出处
《第四军医大学学报》
北大核心
2006年第10期886-889,共4页
Journal of the Fourth Military Medical University
