摘要
利用RT—PCR技术从纽荷尔脐橙果实eDNA中分离出辣椒红／辣椒玉红素合成酶（Capsanthin／Capsorubin synlhase，CCS）基因（Ccs）全长。该片断长为1619bp，编码503个氨基酸.BLAST比较其氨基酸同源性发现，该序列所推导的氨基酸与已知的甜橙CCS完全一致，与胡萝卜和辣椒的CCS，以及马铃薯新黄质合成酶（neoxanthin synthase，NSY）有70％的同源性。此外，与番茄和柑橘等的番茄红素环化酶也有50％～70％的同源性。半定量RT—PCR分析表明，Ccs在纽荷尔脐橙果实成熟过程中呈先上升后下降的表达趋势，期间9月份的表达量最高。
The mRNA sequence for Capsantbin/Capsoruhin synthase （CCS） has been cloned from fi-uits of Newhall navel orange by RT-PCR. Sequencing results revealed that it was 1 619 bp in length, encoding 503 amino acids. BLAST results showed that the deduced amino acids shared the same amino acid sequence as that of Citrus sinensis, 70% identities with CCS of Daueus earota and Capsicum annuum. In addition to 70% identities with neoxanthin synthase （NSY） of Solanum tuberosum, it showed 50% -70% identities with lycopene eyelase of Lycopersicon esculentum and citrus. Semi-quantitative RT-PCR results showed that expressions of Ccs were down-regulated during fruit ripening of Newhall navel orange, with the highest one in September.
出处
《植物研究》
CAS
CSCD
北大核心
2008年第5期579-583,共5页
Bulletin of Botanical Research
基金
国家自然科学基金(30300241)
湘潭大学科研启动费项目(06QDZ11)
湘潭大学校级交叉项目(06IND01)资助
