摘要
目的:建立一种快速、敏感、特异性高的麻疹病毒核酸检测方法,以便对早期麻疹病毒感染做出准确及时的临床诊断。方法:采用TaqMan特异性荧光标记探针标记的RT-PCR技术对40例临床疑似麻疹患者的咽拭子进行麻疹病毒核酸的检测,同时对其血清用酶联免疫吸附试验(ELISA)检测麻疹特异性抗体IgM。结果:40份患者咽拭子RT-PCR检测均阳性,40位患者入院第2天血清ELISA法检测,麻疹特异性抗体IgM阳性28例,阴性12例,1周后对12例麻疹特异性抗体IgM阴性的患者再次检测,结果12例全阳性。结论:荧光定量RT-PCR检测麻疹病毒方法有较好的敏感性和特异性,与血清麻疹特异性抗体IgM检测方法相比,荧光定量RT-PCR法在临床症状出现后就能检测到,同时不受标本量的限制,在短时间内就可完成检测,更适合作麻疹早期诊断的实验室检查依据。
Objective：To establish a quick,sensitive and specific detection method to diagnose the infection of measles virus in initial stage.Methods：Measles virus was isolated from throat swab specimens collected from 40 suspected measles cases and nucleinic acid of measles virus was amplified by TaqMan-based real-time RT-PCR assay.At the same time,serum specimen from 40 suspected measles cases were detected measles IgM by ELISA.Results：The 40 throat swab specimens were all positive by RT-PCR assay.And 28 serum specimens were positive measles IgM by ELISA,and other 12 serum specimens were negative measles IgM.However,other 12 serum specimens with negative measles IgM changed to be positive by ELISA after a week.Conclusion：Compared with method of detection of serum measles IgM,fluorescent quantitation RT-PCR is more sensitive and specific.Fluorescent quantitation RT-PCR assay can be used to rapidly diagnose measles cases without limited by specimen quantitation,especially to those patients with rash in the initial stage.
出处
《中国卫生检验杂志》
CAS
2008年第9期1797-1798,共2页
Chinese Journal of Health Laboratory Technology
