摘要
探讨不同结构丝素蛋白对羟基磷灰石结晶的影响,可为羟基磷灰石/丝素蛋白复合材料应用于骨组织工程支架材料的研究提供依据。用中性盐水解的方法获得以无规卷曲结构为主的丝素蛋白(SF),再经乙醇处理得到具有β-折叠结构的丝素蛋白(ALSF)。以两种不同结构的丝素蛋白作为羟基磷灰石(HA)沉积的有机模板,通过共沉淀的方法获得HA/SF和HA/ALSF复合粉末。利用红外吸收光谱(FTIR)、X-射线衍射(XRD)、透射电子显微镜(TEM)等对复合材料样品进行检测和分析表明,2种丝素蛋白均对HA晶体的形核和生长产生影响,但不同结构丝素蛋白调控生成的复合材料其形貌和结晶尺寸有所不同。
In this paper, we investigated the effect of different structure silk fibroin on crystallization of hydroxyapatite （HA）, so as to provide the evidences for HA/silk fibroin composite used as a scaffold in the bone tissue engineering. A kind of silk fibroin （SF） with coil/helical structure was prepared through neutral salt dissolution method, and the other kind of silk fibroin （ALSF） with β-sheet structure was obtained from the former SF by treatment of alcohol solution. HA/SF and HA/ALSF composites were respectively deposited with the organic template of those two silk fibroin. The second structure, phase composition and morphology of HA, HA/ SF and HA/ALSF composites were investigated by Fourier transform infrared spectroscopy（FTIR）, X-ray diffraction（XRD） and transmission electron microscopy（TEM）. These results showed that nucleation and growth of HA were affected by silk fibroin structure, which induced different morphology and particle size of HA.
出处
《蚕业科学》
CAS
CSCD
北大核心
2008年第3期472-476,共5页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金项目(编号30771633)
关键词
羟基磷灰石
丝素蛋白结构
复合材料
晶体形貌
晶体尺寸
Hydroxyapatite
Silk fibroin structure
Composite material
Crystal morphology
Crystal size
