摘要
目的探讨健康大鼠中耳黏膜中液体吸收速度和钠离子浓度的相关性。方法24只大鼠分成Ⅰ、Ⅱ、Ⅲ、Ⅳ组,每组6只,全身麻醉后分别在各组实验耳注入10、40、80、140 mM的NaCl溶液,对照耳注入140 mM NaCl溶液。30分钟后,中耳腔液体的体积开始发生稳定变化,监测液体体积的变化,描记体积和时间变化曲线,通过线性回归计算液体吸收速率(K);吸收速率比(KR=K实验/K对照)定义为实验耳的吸收速率(K实验)与对照耳的吸收速率(K对照)的比值。结果Ⅰ、Ⅱ、Ⅲ、Ⅳ组的KR值分别为:0.5455±0.0685、0.6353±0.0579、0.7575±0.0611、0.9774±0.0585,差异有显著统计学意义(P<0.001);通过线性回归分析,KR值和钠离子浓度呈正相关性(r=0.9424,P<0.001)。结论本实验证实了活体动物中耳黏膜具有吸收功能,其吸收速度与钠离子的浓度呈正相关。
Objective To investigated the correlation between the fluid transport and sodium concentration across the middle ear mucous in healthy rats. Methods 24 rats were divided into 4 groups and then maintained under general anesthesia. One of the bulla was filled with sodium solutions of 10,40, 80 and 140 mM,respectively, while the other side with 140 mM sodium solution. After 30 minutes, the fluid volume change became stabilized and the variations were continuously measured. The changes expressed in the voume/time curve were plotted. The absorption rate was calculated by fitting linear regression. The absorption ratio was defined as the absportion rate of the control side divided by that of experimental side. Results The regression analysis indecated that the KR values were：KRⅠ0. 5455±0. 0685,KRⅡ0. 6353±0. 0579,KRⅢ0. 7575±0. 0611 ,KRⅣ0. 9774±0. 0585 （P〈0. 001）. The KR value was positively correlated with the sodium concentration（r=0. 9424,P〈0. 001）. Conclusion This experiment confirmed that the positive correlation between the fluid absorption rate and the sodium concentration, and further demonstrated the absorption function of the middle ear mucous in rats.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2008年第5期401-404,共4页
Journal of Audiology and Speech Pathology
基金
浦东新区社会发展局资助项目(pw2005D-3)
关键词
中耳
上皮钠通道
钠离子
大鼠
Middle ear
Epithelium sodium channel
Sodium ion
Rat
