摘要
目的：建立HPLC梯度洗脱法测定天山花楸叶中芦丁含量的方法。方法：采用ZORBAX SB-C18（4.6mm×15mm,5μm）色谱柱,甲醇-0.02%磷酸溶液为流动相（梯度洗脱）,流速为1.0ml·min^-1,检测波长为326nm。结果：芦丁线性范围为0.2104-1.8936μg/ml,叶中芦丁含量0.4829mg/100ml（RSD%=0.48%,n=3）,平均回收率为100.4%。结论：该方法经济、简便、快速、准确、可靠,可用于该药材的质量控制。
Objecthe： To establish the method of Rutin determination in Sorbus Tiansehaniea. Methods： ZORBAX SB - C18 （4.6mm ×15mm, 5μm） column of High Performance Liquid Chromatography （ HPLC ） was adopted in the Rutin determination. The MeOH-0.02% H3PO3 was used as mobile phase. The flow rate was 1.0ml·min^-1. The detective wavelength was 326nm. Results： The linear range of Rutin was 0.2104 - 1.893μg/ml. Rutin in the leaf of S. Tianschanica was 0.4829mg/100ml （RSD% = 0.48%, n = 3）, and the average recovery- rate was 100.4%. Conclusion： The method is simple and sensifive, and can determine Rutin content quickly and correctly. It is suitable for the analysis of Rutin in S. Tiansehanica.
出处
《四川中医》
北大核心
2008年第9期51-53,共3页
Journal of Sichuan of Traditional Chinese Medicine
基金
新疆生产建设兵团科技攻关计划课题(2006GG52)。
