摘要
目的建立大鼠心肌梗死后心力衰竭模型,完善制作方法。方法雄性SD大鼠随机分为冠脉结扎实验组和假手术组(不结扎冠脉),同时记录心电图。术中通过经口气管插管和呼吸机给予正压通气,维持呼吸。术后4周经右颈总动脉插管进行血流动力学检查,检测心率(HR)、左心室舒张末压(LVEDP)、左室压力最大上升速率(+dp/dtmax)和左室压力最大下降速率(-dp/dtmax)。检测完毕处死大鼠,取右心室及肺组织称重,并与体重相比,HE染色法确定心肌梗死面积。结果左冠状动脉结扎后存活4周的大鼠LVEDP平均水平为-5.85 mmHg,显著高于假手术组(P<0.05),LV+dp/dtmax及LV-dp/dtmax显著低于假手术组(P<0.05)。右心/体重比和肺/体重比均高于假手术组(P<0.05)。病理学检测可见实验组心肌出现梗死区。结论实验组大鼠心肌收缩及舒张功能明显受损,心力衰竭模型制作成功。经口气管插管维持通气的方法简化了操作,降低了手术风险,更利于术后大鼠存活。
Objective To explore a better method for establishing model of congestive heart failure（CHF）in rats after myocardial infarction. Methods The male SD rats were randomly divided into two groups： control group and ligation group. Rats in ligation group underwent coronary ligation to induce CHF, confirmed by electrocardiogram, while the rats in control group underwent thoracotomy without coronary ligation. After 4 weeks, rats were anesthetized and underwent hemodynamic measurement（ HR, LVEDP, ＋ dp/dt max, - dp/dtmax）. Then the fight ventricle and lungs were harvested, and fight ventricular weight and wet lung weight, with respect to body weight, were measured as indices of pulmonary congestion and right ventricular remodeling. The area of infarction was also measured by HE staining. Results Compared with control group, left ventricular end-diastolic pressure, fight ventricle/body weight and lung/body weight ratios were higher in ligation group（ P 〈 0.05 ）. On the contrary, LV ＋ dp/dtmax and LV - dp/dtmax were lower after coronary were ligated（P 〈 0. 05）. Conclusion Coronary ligation can induce congestive heart failure model. The method by using a trachea cannula through mouth to maintain breath is simple, convenient and with lower operation risk, and can improve the survival rate of rat after coronary ligation.
出处
《山西医科大学学报》
CAS
2008年第9期769-771,共3页
Journal of Shanxi Medical University
基金
山西省国际科技合作计划基金资助项目(2006081024)
