摘要
目的 :筛选东方田鼠特异性杀伤日本血吸虫童虫的组织 器官。方法 :将日本血吸虫童虫与东方田鼠心脏、肝脏、肺脏、肾脏、脾脏和肌肉的组织 器官匀浆上清液以及不同浓度东方田鼠血清混合培养 ,以昆明小鼠相应的组织 器官匀浆上清及相应浓度的血清做为对照 ,观察其杀童虫效应。结果 :东方田鼠各组织 器官与昆明小鼠各组织 器官匀浆的杀伤童虫效应比较差异均无显著性 (P >0 .0 5 ) ;东方田鼠血清较昆明小鼠血清的杀伤效应强 (P =0 .0 0 1) ;东方田鼠各组织 器官匀浆之间杀伤效应差异亦无显著性 (P >0 .0 5 ) ;东方田鼠血清较东方田鼠各组织 器官的杀伤效应强 (P <0 .0 5 ) ;东方田鼠新鲜血清与灭活补体血清的杀伤效应无显著性差异 (P >0 .0 5 ) ,其 4 0 %新鲜血清较2 0 %新鲜血清的杀伤效应强 (P <0 .0 0 1)。结论 :东方田鼠血清较组织 器官的杀伤效应强 ;也较昆明小鼠血清的杀伤效应强 ,东方田鼠 4 0 %血清浓度较 2 0
Objective To screen the specific tissues/organs of Microtus fortis (MF) that have the activity of anti schistosomula of Schistosoma japonicum. Methods The homogenate supernatant of the heart, liver, lung, spleen, kidney, muscle and fresh serum of MF and those of mice were mixed with schistosomula respectively and cultured at 37℃ with 5% CO 2. The schistosomula killing effect of Schistosoma japonicum was observed. Results There was no significant difference between the tissues/organs of MF and those of the mice in schistosomula killing effect (P>0.05), as it was the same with the tissues/organs of MF (P>0.05) and between the fresh serum of MF and its complement inactivated serum (P>0.05). The schistosomula killing effect of the serum of MF was stronger than that of the mice (P=0.001), and the same compared to the tissues/organs of MF (P<0.05). Between the 40% fresh serum of MF and its 20% fresh serum, there was a significant difference (P<0.001). Conclusion The schistosomula killing effect of the serum of MF was stronger than that of mice, and it was the same with the tissues/organs of MF.
出处
《湖南医科大学学报》
CSCD
北大核心
2002年第3期198-200,共3页
Bulletin of Hunan Medical University
基金
"九 .五"国家科技攻关项目 (编号 :96-A2 3 -0 6-0 4)
湖南省科技厅"九.五"科技攻关配套基金
国家自然科学基金 (批准号 :3 0 0 70 40 3 )资助
