摘要
[目的]对微波联合大孔树脂提取腊梅花总黄酮的新工艺进行研究。[方法]在不同的提取工艺条件下进行平行试验、正交试验和对比试验。[结果]原料0.500 g的腊梅花粉末,以70%的乙醇溶液作提取剂,提取剂的用量为1∶60(g/ml),微波功率为288 W,在提取时间为450 s下提取次数3为最佳工艺。确定AB-8为腊梅花总黄酮的吸附树脂。其最佳静态吸附工艺条件为:温度30℃,吸附时间3 h;最佳动态解吸工艺条件为:流速2.6 ml/min,上样液pH值4.3,30 ml 70%乙醇洗脱。与溶剂浸提法相比,采用微波法提取腊梅花总黄酮提取时间由20 h缩短为450 s,提取率从79.86%提高到92.23%,而且AB-8分离腊梅花总黄酮分离效果明显,成本低。[结论]微波联合大孔树脂提取腊梅花总黄酮可工业化推广。
[ Objective ] The research aimed to study a new method of microwave joint macroreticular resin extracting flavonoids from Chimonanthus praecox （L.） Link.. [Method] Under different extraction conditions, the authors made parallel test, orthogonal test and contrast test. [Result]The optimal condition was as follow： Raw materials was 0.500 g Chimonanthus praecox （L.） Link. dry powder, the extraction agent was 70% alcohol solution, the ratio of raw material to extraction agent was 1：60 （g/mi）, the microwave power was 288 W, and the extraction time was 450 s for three times. AB-8 was determined as the absorption resin to total flavonoids of Chimonanthus praecox （L.） Link.. The best passive absorption process conditions was as follow： Temperature was 30℃, adsorption time was 3 h. The best dynamic desorption process was as follow： Velocity was 2.6 ml/min, pH value of the upper liquid was 4.3, 30 ml of TO percent ethanol was used to clear. Compared with solvent extraction, the extraction time of microwave extraction was reduced from 20 h to 450 s, the extraction rate was increased from 79.86% to 92.23%, and that AB-8 was used to extract Chimonanthus praecoc （L.） Link. flavonoids were very effective and low cost. [ Conclusion ] microwave joint macroreticular resin extraction flavonoids from Chiraonanthus praecox （ L. ） Link. can promote industrialization.
出处
《安徽农业科学》
CAS
北大核心
2008年第25期10734-10736,共3页
Journal of Anhui Agricultural Sciences
基金
广东省自然科学基金资助课题(06023571)
关键词
微波提取
腊梅花
大孔树脂
总黄酮
Microwave extraction
Chimonanthus praecox （L.） Link.
Macroreticular resin
FLavonoids
