摘要
采用假阴道法采集成年种公驴精液,经品质评定后,用5种不同冷冻保存液稀释、平衡,冷冻保存制作细管冷冻精液,以筛选适宜的驴精液冷冻保存液和冷冻保护剂。结果表明:乳糖-葡糖-甘油-卵黄液为最佳种冷冻保存液(P<0.05);甘油为最佳冷冻保护剂,其适宜浓度为5%,精子冻后活率平均为0.36,畸形率≤27.4%,顶体完整率82.2%,解冻后常温下精子存活时间为8.2h。
The donkey semen was collected by artificial vagina. The extenders of frozen semen, cryoprotectant and the glycerol concentration were investigale in present study. The results shows that the lactose-glucose-glycerol-yolk extender was the best one in 5 groups （P〈0.05）. The Glycerol was the better cryoprotective preservative compared with Glycol and DMSO. The optimum Glycerol concentration is 5%（P〈0.05）. The sperm motility after-thawing was the highest in 5 treatment groups. The semen motility of frozen thawing was up to 0.36, Abnormal sperm after-thawing was 27.4%, acrosome integrity after-thawing was 82.2%, and sperm survival time（25℃） was8.2 h, respectively.
出处
《草食家畜》
2008年第3期18-20,共3页
Grass-Feeding Livestock
基金
新疆维吾尔自治区科技攻关项目<肉乳兼用驴繁殖与饲养技术开发和示范>资助
编号为200731103
关键词
驴
精液
冷冻保存
冷冻保护剂
donkey
semen
freezing preservation
freezing preservative
