摘要
目的:探讨冷冻方法和冷冻保护剂类型对精子活率和尾部低渗肿胀率的影响。方法:16例男性不育患者精液分别于冷冻前、加入甘油卵黄枸橼酸钠(GYC)和甘油两种冷冻液以快速和慢速冷冻6个月后,采用伊红染色分析精子活率和尾部低渗肿胀率。结果:冷冻后精子活率和尾部低渗肿胀率明显低于冷冻前(P<0.001,P<0.05);GYC和甘油两种冷冻保护剂对精子活率和尾部低渗肿胀率的影响差异无显著性(P>0.05);速冻和缓慢冷冻对精子活率和尾部低渗肿胀率的影响差异亦无显著性(P>0.05)。结论:冷冻后精子活率和尾部低渗肿胀率明显降低,不同冷冻方法和冷冻保护剂类型对精子活率和尾部低渗肿胀率无影响。
Objective To study the effects of cryopreservative methods and cryopreservative mediums on hypotonic swelling rate of human spermatozoa tail and sperm viability. Methods Human semen samples from 16 male patients with infertility were cryopreserved by fast frozen and slow frozen methods. The hypotonic swelling rate of human spermatozoa tail and sperm viability were analyzed by Eosin staining. Results Post-thawing the spermatozoa tail hypotonic swelling rate and sperm viability were significantly lower than that before (P<0.05, P<0.001), whereas there were no statistical differences in the hypotonic swelling rate of human spermatozoa tail and sperm viability between fast frozen and slow frozen(P>0.05). There were no statistical differences in the hypotonic swelling rate of human spermatozoa tail and sperm viability between GYC and glycerol groups (P>0.05).Conclusion Cryopreservation induces the descent of hypotonic swelling rate of human spermatozoa tail and sperm viability. Cryopreservative methods and cryoprotective mediums could not affect hypotonic swelling rate of human spermatozoa tail and sperm viability.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2005年第4期605-607,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题(20030433)
关键词
精液保存
精子尾
低渗肿胀率
冷冻保护剂
精子活率
semen preservation
sperm tail
hypotonic swelling rate
cryoprotective mediums
sperm viability
