摘要
目的：研究子痫前期患者外周血中性粒细胞凋亡情况及其机制。方法：选取2003年10月-2004年9月在我院产科住院的子痫前期患者、正常妊娠孕妇各20例，分离外周血中性粒细胞并体外培养24h，流式细胞术检测凋亡率，RT-PCR检测Mcl-1mRNA的表达，免疫印迹法检测caspase-3蛋白的表达。结果：子痫前期组外周血中性粒细胞凋亡率（16．51％±3．84％）明显低于正常妊娠组（30．62％±6．27％），子痫前期组中性粒细胞Mcl-1mRNA表达增加，caspase-3蛋白活化降低。结论：子痫前期患者外周血中性粒细胞存在凋亡延迟，Mcl-1表达上调和caspase-3活化抑制可能是凋亡延迟的重要机制。
Objective： To investigate peripheral neutrophil apoptosis in preeclampsia patients and its mechanism. Methods： Neutrophils isolated from 20 preeclamptic patients and 20 normal pregnancies were incubated with autologous serum in vitro for 24 h. Then the ap- optosis rate was determined by Flow Cytometry, and Mcl - 1 mRNA expression was determined by RT - PCR, caspase - 3 protein expression was determined by Western blotting. Results： Apoptosis rate was significantly reduced in neutrophils from preeclampfic patients （16. 51% ± 3. 84% ） compared with that of normal pregnancies （30. 62% ± 6. 27% ）. In precclamptic group, Mcl - 1 mRNA expression elevated, but caspase - 3 protein expression decreased. Conduslon： Apoptosis in peripheral neutrophil of preeclampsia delays, the upregulation of Mcl - 1 and downregulation of caspase - 3 may act an important role in the apotosis delay.
出处
《中国妇幼保健》
CAS
北大核心
2008年第27期3881-3883,共3页
Maternal and Child Health Care of China
基金
国家自然科学基金(NO.30371751)
