摘要
目的研究急性早幼粒白血病（ＡＰＬ）细胞株ＮＢ４细胞ＰＭＬ－ＲＡＲα（早幼粒细胞白血病基因－维甲酸受体基因α）融合基因的表达对维甲酸诱导分化作用的影响，探讨ＰＭＬ－ＲＡＲα融合基因与维甲酸治疗作用间的关系。方法用反义核酸来阻断ＰＭＬ－ＲＡＲα融合基因的表达并用逆转录－聚合酶链式反应检测。ＮＢ４细胞的生长、分化及功能。通过细胞生长曲线、形态学、细胞表面标志及ＮＢＴ（硝基四氮唑蓝）还原试验进行判定，细胞周期应用流式细胞仪进行分析。结果反义核酸阻断ＰＭＬ－ＲＡＲα表达后使维甲酸对ＮＢ４细胞的生长抑制作用增强，Ｓ期细胞由５２％降至２９％，同时ＮＢＴ还原能力提高，细胞表面标志变化明显，提示对维甲酸敏感性增加。结论ＰＭＬ－ＲＡＲα融合基因的表达不仅与ＡＰＬ的发病有关，而且钝化了维甲酸对ＡＰＬ细胞的诱导分化作用。
bjective To study the relation between the PML RARα gene and the effects of ATRA on proliferation and differentiation in acute promyelocytic leukemia cell line NB4 cells. Methods ASODN mediated inactivation of the PML RARα mRNA was measured by RT PCR. The proliferation and differentiation of NB4 cells were determined by proliferation curve, morphology, CD antigen of membrane and NBT test. NB4 Cell cycle was analyzed by FACS. Results The inactivation of PML RARα mRNA by ASODN could egnhance the maturation effects and growth suppression of ATRA on NB4 cells. The percentage of S phase cell was decreased from 52% to 29%, and NBT rate increased. All suggest that the sensitivity of NB4 cell to ATRA was improved. Conclusion The PML RARα gene, as a molecular marker of APL, is responsible for pathogenesis of APL, and it also decreases the sensitivity of ATRA to APL cells.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第10期745-749,共5页
National Medical Journal of China
基金
国家自然科学基金
关键词
白血病
维甲酸
APL
PML-RARΑ
融合基因
Gene expression regulation, leukemic Retinoic acid Leukemia, promyelocyttic, acute
