摘要
采用毛细管气相色谱法对９种缓慢生长分枝杆菌的化学成分进行了分析。结果表明，不同菌种的色谱图存在明显的差异。对７５株临床菌株进行色谱法鉴别，并与传统生物学鉴定法作了对比。
A method for the characterization of mycobacteria species by capillary gas chromatography is described. After strains of mycobacteria being hydrolyzed, saponified in an acidic solution, various methyl esters resulted from mycobacteria were studied on an OV101 column at optimum chromatographic conditions. The constituents of 9 slowlygrowing mycobacteria species were analyzed and the results showed that there were obvious differences between different mycobacteria species, but same characteristics were observed between strains of the same species. The 9 species can be identified by the ratio of peak height of C 18∶1 , C 18∶0 and TBSA. They are C 18∶1 >TBSA>C 18∶0 for M.tuberculosis, C 18∶0 >C 18∶1 >TBSA for M.triviale, TBSA>C 18∶1 >C 18∶0 for M.intracellulare, TBSA>C 18∶0 >C 18∶1 for M.avium and C 18∶1 >C 18∶0 >TBSA for M.bovis, M.kansasii, M.gordonae, M.nonchromogenicum and M. terrae respectively. The 5 latter species can be distinguished by the presence of characteristic peaks of iC 16∶1 and C 19∶0 . 75 strains of clinical isolated mycobacteria were analysed at the same chromatographic conditions. The results showed that they belonged to 9 different species. The accuracy of the proposed method was examined according to biological analysis.
出处
《色谱》
CAS
CSCD
北大核心
1997年第6期502-504,共3页
Chinese Journal of Chromatography
关键词
毛细管气相色谱
分枝杆菌种属
临床应用
TBSA
capillary gas chromatography, the constituents of mycobacteria species, clinical analysis
