摘要
使用弹性石英毛细管气相色谱(固定相OV—101)法,研究了十八种蛋白质氨基酸的衍生条件,选择了较理想的酯化和酰化条件,在二十分钟内成功地分离分析了十八种蛋白质氨基酸,其中大多数氨基酸分析的相对误差<5%。
In this paper,the derivative conditions for eighteen kinds of protein amino acids are studied by the gas chromatography of quartz capillary.With this method,the perfect conditions of esterification and acylication are chosen and the eighteen kinds of amino acids are separated and analyzed only in twenty minutes.The relative errors of our results are fewer than 5%.
关键词
氨基酸
毛细管气相色谱
酯化
酰化
分析
amino,capillary gas chromatography,esterification,acylication